Product Details
| Alternative Name: | NF-κB inhibitor α, NFKBIA |
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| Clone: | 6A920 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant human IκBα (aa 32-391). |
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| UniProt ID: | P25963 |
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| Species reactivity: | Human, Mouse
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| Specificity: | Recognizes both phosphorylated and non-phosphorylated forms of IκBα. |
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| Applications: | Flow Cytometry, IHC (PS), IP, WB
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| Recommended Dilutions/Conditions: | Flow cytometry (0.25-1µg/106cells)
Immunoprecipitation (1µg/ml)
Western Blot (1-2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~40kDa by Western blot. |
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| Positive Control: | Whole cell lysate from Jurkat cells can be used as positive control. |
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| Purity Detail: | Protein G affinity-purified. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for 6 months when stored at +4°C. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of IkBa using Prod. No. ALX-804-209 at 2µg/ml in (A) Daudi and (B) NIH 3T3 whole cell lysate.
Intracellular staining of 106 Thp-1 cells using 0.25µg of IκBα, mAb (6A920) (Prod. No. ALX-804-209).
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Product Literature References
BRAFV600E mutation, TIMP-1 upregulation, and NF-kappaB activation: closing the loop on the papillary thyroid cancer trilogy: A. Bommarito, et al.; Endocr. Relat. Cancer
18, 669 (2011),
Abstract;
Full Text
Adenoviral expression of XIAP antisense RNA induces apoptosis in glioma cells and suppresses the growth of xenografts in nude mice: U. Naumann, et al.; Gene Ther.
14, 147 (2007),
Abstract;
NF-kappaB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins: W.S. Santhi, et al.; Exp. Mol. Pathol.
81, 123 (2006), Application(s): IHC (paraffin sections),
Abstract;
General Literature References
Modulation of NF-kappaB activity by exchange of dimers: S. Saccani, et al.; Mol. Cell
11, 1563 (2003),
Abstract;
PC-SPES: a potent inhibitor of nuclear factor-kappa B rescues mice from lipopolysaccharide-induced septic shock: T. Ikezoe, et al.; Mol. Pharmacol.
64, 1521 (2003),
Abstract;
p38-Dependent marking of inflammatory genes for increased NF-kappa B recruitment: S. Saccani, et al.; Nat. Immunol.
3, 69 (2002),
Abstract;
Two waves of nuclear factor kappaB recruitment to target promoters: S. Saccani, et al.; J. Exp. Med.
193, 1351 (2001),
Abstract;
Full Text
