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IκBα monoclonal antibody (6A920)

ALX-804-209-C100 100 µg 464.00 USD
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Product Details

Alternative Name:NF-κB inhibitor α, NFKBIA
Immunogen:Recombinant human IκBα (aa 32-391).
UniProt ID:P25963
Species reactivity:Human, Mouse
Specificity:Recognizes both phosphorylated and non-phosphorylated forms of IκBα.
Applications:Flow Cytometry, IHC (PS), IP, WB
Recommended Dilutions/Conditions:Flow cytometry (0.25-1µg/106cells)
Immunoprecipitation (1µg/ml)
Western Blot (1-2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~40kDa by Western blot.
Positive Control:Whole cell lysate from Jurkat cells can be used as positive control.
Purity Detail:Protein G affinity-purified.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Use/Stability:Stable for 6 months when stored at +4°C.
Shipping:Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
IκBα monoclonal antibody (6A920) Western blot
Western blot analysis of IkBa using Prod. No. ALX-804-209 at 2µg/ml in (A) Daudi and (B) NIH 3T3 whole cell lysate.
IκBα monoclonal antibody (6A920) Flow Cytometry
Intracellular staining of 106 Thp-1 cells using 0.25µg of IκBα, mAb (6A920) (Prod. No. ALX-804-209).
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IκBα monoclonal antibody (6A920) Western blot IκBα monoclonal antibody (6A920) Flow Cytometry

Product Literature References

BRAFV600E mutation, TIMP-1 upregulation, and NF-kappaB activation: closing the loop on the papillary thyroid cancer trilogy: A. Bommarito, et al.; Endocr. Relat. Cancer 18, 669 (2011), Abstract; Full Text
Adenoviral expression of XIAP antisense RNA induces apoptosis in glioma cells and suppresses the growth of xenografts in nude mice: U. Naumann, et al.; Gene Ther. 14, 147 (2007), Abstract;
NF-kappaB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins: W.S. Santhi, et al.; Exp. Mol. Pathol. 81, 123 (2006), Application(s): IHC (paraffin sections), Abstract;

General Literature References

Modulation of NF-kappaB activity by exchange of dimers: S. Saccani, et al.; Mol. Cell 11, 1563 (2003), Abstract;
PC-SPES: a potent inhibitor of nuclear factor-kappa B rescues mice from lipopolysaccharide-induced septic shock: T. Ikezoe, et al.; Mol. Pharmacol. 64, 1521 (2003), Abstract;
p38-Dependent marking of inflammatory genes for increased NF-kappa B recruitment: S. Saccani, et al.; Nat. Immunol. 3, 69 (2002), Abstract;
Two waves of nuclear factor kappaB recruitment to target promoters: S. Saccani, et al.; J. Exp. Med. 193, 1351 (2001), Abstract; Full Text

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