Product Details
| Alternative Name: | Uvomorulin, CD324, Epithelial cadherin, Cadherin-1 |
| |
| Clone: | HECD-1 |
| |
| Host: | Mouse |
| |
| Isotype: | IgG1 |
| |
| Immunogen: | Human breast tumor cell line MCF-7. |
| |
| UniProt ID: | P12830 |
| |
| Source: | Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of BALB/c mouse immunized with human breast tumor cell line MCF-7. |
| |
| Species reactivity: | Human
Guinea pig
|
| |
| Crossreactivity: | Does not cross-react with rat, dog, bovine or chicken E-cadherin. |
| |
| Applications: | ELISA, Flow Cytometry, ICC, IHC (FS), IHC (PS), WB, FUNC
|
| |
| Recommended Dilutions/Conditions: | ELISA (2-10µg/ml)
Flow Cytometry (1µg/ml)
Immunocytochemistry (1µg/ml)
Immunohistochemistry (frozen sections (2-10µg/ml)
paraffin sections (2-10µg/ml))
Western Blot (2-10µg/ml)
Functional Application (100µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
| |
| Application Notes: | Functional Application: inhibits of E-cadherin dependent cell-cell contact. |
| |
| Purity Detail: | Ammonium sulfate precipitation and ion exchange chromatography. |
| |
| Formulation: | Lyophilized from 10mM PBS, pH 7.4 , containing 1% BSA. Contains no preservatives. |
| |
| Reconstitution: | Reconstitute with 50µl distilled water. Dilute with 20mM TBS, pH 7.4, 10mM CaCl2, 1.0% BSA. Reconstituted solution should contain 0.1% sodium azide as a preservative when stored at +4°C. |
| |
| Use/Stability: | The stock solution should be stored in aliquotes at -20°C for 1 year or should be stored at 4°C for 6 months after adding 0.1% sodium azide. |
| |
| Handling: | Dilute only prior to immediate use. Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
| |
| Shipping: | Blue Ice Not Frozen |
| |
| Long Term Storage: | +4°C |
| |
| Technical Info/Product Notes: | Ca2+ should be contained in all buffers to stabilize cadherin antigen. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
In vitro segregation and isolation of human pluripotent stem cell-derived neural crest cells: S. Münst, et al.; Methods
133, 65 (2018),
Abstract;
Leucine-rich repeat-containing G protein-coupled receptor 5 regulates epithelial cell phenotype and survival of hepatocellular carcinoma cells: M. Fukuma, et al.; Exp. Cell Res.
319, 113 (2013),
Abstract;
Biomarker analysis of cetuximab plus oxaliplatin/leucovorin/5-fluorouracil in first-line metastatic gastric and oesophago-gastric junction cancer: results from a phase II trial of the Arbeitsgemeinschaft Internistische Onkologie (AIO): B. Luber, et al.; BMC Cancer
11, 509 (2011),
Abstract;
Full Text
Changing etiology of tubal pregnancy following IVF: A. Revel, et al.; Hum. Reprod.
6, 1372 (2008),
Abstract;
CD123bright plasmacytoid predendritic cells: progenitors undergoing cell fate conversion?: M.R. Comeau, et al.; J. Immunol.
169, 75 (2002),
Abstract;
Full Text
Differential expression of E-cadherin in normal, metaplastic and dysplastic esophageal mucosa - a putative biomarker: J.A. Jankowski, et al.; Int. J. Oncol.
4, 441 (1994),
Abstract;
Immunohistochemical localization of cell adhesion molecule epithelial cadherin in human arachnoid villi and meningiomas: Y. Tohma, et al.; Cancer Res.
52, 1981 (1992),
Abstract;
Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections: S.R. Shi, et al.; J. Histochem. Cytochem.
39, 741 (1991),
Abstract;
Cadherin cell adhesion receptors as a morphogenetic regulator: M. Takeichi; Science
251, 1451 (1991),
Abstract;
Cadherin intercellular adhesion molecule in hepatocellular carcinomas: loss of E-cadherin expression in an undifferentiated carcinoma: Y. Shimoyama & S. Hirohashi; Cancer Lett.
57, 131 (1991),
Abstract;
Expression of E- and P-cadherin in gastric carcinomas: Y. Shimoyama & S. Hirohashi; Cancer Res.
51, 2185 (1991),
Abstract;
Expression of immunoreactive E-cadherin adhesion molecules in human cancers: H. Shiozaki, et al.; Am. J. Pathol.
139, 17 (1991),
Abstract;
Cadherin cell-adhesion molecules in human epithelial tissues and carcinomas: Y. Shimoyama, et al.; Cancer Res.
49, 2128 (1989),
Abstract;
