Product Specification
| Alternative Name: | Uvomorulin, CD324, Epithelial cadherin, Cadherin-1 |
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| Clone: | HECD-1 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Human breast tumor cell line MCF-7. |
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| UniProt ID: | P12830 |
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| Source: | Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of BALB/c mouse immunized with human breast tumor cell line MCF-7. |
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| Species reactivity: | Human
Guinea pig
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| Crossreactivity: | Does not cross-react with rat, dog, bovine or chicken E-cadherin. |
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| Applications: | ELISA, Flow Cytometry, ICC, IHC (FS), IHC (PS), WB, FUNC
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| Recommended Dilutions/Conditions: | ELISA (2-10µg/ml)
Flow Cytometry (1µg/ml)
Immunocytochemistry (1µg/ml)
Immunohistochemistry (frozen sections (2-10µg/ml)
paraffin sections (2-10µg/ml))
Western Blot (2-10µg/ml)
Functional Application (100µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Functional Application: inhibits of E-cadherin dependent cell-cell contact. |
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| Purity Detail: | Ammonium sulfate precipitation and ion exchange chromatography. |
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| Formulation: | Lyophilized from 10mM PBS, pH 7.4 , containing 1% BSA. Contains no preservatives. |
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| Reconstitution: | Reconstitute with 50µl distilled water. Dilute with 20mM TBS, pH 7.4, 10mM CaCl2, 1.0% BSA. Reconstituted solution should contain 0.1% sodium azide as a preservative when stored at +4°C. |
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| Use/Stability: | The stock solution should be stored in aliquotes at -20°C for 1 year or should be stored at 4°C for 6 months after adding 0.1% sodium azide. |
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| Handling: | Dilute only prior to immediate use. Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Technical Info/Product Notes: | Ca2+ should be contained in all buffers to stabilize cadherin antigen. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Full Text
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Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections: S.R. Shi, et al.; J. Histochem. Cytochem.
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