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5-Bromodeoxyuridine monoclonal antibody (PRB-1) (fluorescein conjugate)

ALX-804-197L-T060 60 tests Fluorescein 386.00 USD
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Product Details

Alternative Name:BrdU
Immunogen:IU- (iodouridine-) conjugated ovalbumin.
Species reactivity:Species independent
Specificity:Recognizes BrdU (5-bromodeoxyuridine) and IU either free, within single-stranded DNA, or coupled to a protein carrier.
Crossreactivity:Cross-reacts with iodouridine. Does not cross-react with thymidine.
Applications:Flow Cytometry, ICC
Recommended Dilutions/Conditions:Flow Cytometry (1:20)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Formulation:Liquid. In 300µl PBS, pH 7.4, containing 0.05% sodium azide.
Handling:Do not freeze. Protect from light.
Shipping:Blue Ice
Long Term Storage:+4°C
Scientific Background:5-Bromodeoxyoridine (BrdU) is an analog of thymidine (derivative of uridine) and can be incorporated specifically into DNA in place of thymidine. Cells can be pulse-labelled with BrdU, and those cells that are synthesizing DNA (in S-phase of the cell cycle) will incorporate BrdU into the DNA. MAb to BrdU can then be used to identify cells that undergo DNA synthesis during exposure to BrdU. The proportion of cells in S-phase of the cell cycle can be determined either by fluorescence microspcopy or by flow cytometric analysis.
Regulatory Status:RUO - Research Use Only

Product Literature References

Labelling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation: X. Li & Z. Darzynkiewicz; Cell Prolif. 28, 571 (1995), Abstract;
In vitro BrdUrd incorporation of colorectal tumour tissue: K. Williamson, et al.; Cell Prolif. 26, 115 (1993), Abstract;
Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication: H. G. Gratzner; Science 218, 474 (1982), Abstract;

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