Product Details
| Alternative Name: | Merosin, LAMA2 |
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| Clone: | 4H8-2 |
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| Host: | Rat |
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| Isotype: | IgG1 |
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| Immunogen: | Mouse heart laminin-2 (merosin). |
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| UniProt ID: | Q60675 |
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| Species reactivity: | Human, Mouse
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| Specificity: | Recognizes an N-terminal portion of the α2 chain that is deleted in congenital muscular dystrophies. |
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| Applications: | ELISA, IHC, IP
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| Recommended Dilutions/Conditions: | ELISA (0.02-0.1µg/ml)
Immunoprecipitation (use at 1-10µg/ml in PBS)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Not recommended for Western blot. |
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| Purity Detail: | Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography. |
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| Formulation: | Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose. |
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| Reconstitution: | Reconstitute with 1ml distilled water (15 min, RT). |
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| Use/Stability: | Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The laminins are a family of glycoproteins that provide an integral part of the structural scaffolding of basement membranes in almost every tissue. Each laminin is a heterotrimer assembled from α, β and γ chain subunits, secreted and incorporated into cell-associated extracellular matrices. The laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycans, and other receptors. Through these interactions, laminins critically contribute to cell differentiation, cell shape and movement, maintenance of tissue phenotypes and promotion of tissue survival. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Slc12a8 in the lateral hypothalamus maintains energy metabolism and skeletal muscle functions during aging: N. Ito, et al.; Cell Rep.
40, 111131 (2022),
Abstract;
Mesenchymal Stem Cells Derived From Human Induced Pluripotent Stem Cells Improve the Engraftment of Myogenic Cells by Secreting Urokinase-type Plasminogen receptor (uPAR): A. Elhussieny, et al.; Stem Cell Res. Ther.
12, 532 (2021),
Abstract;
Deficiency of emerin contributes differently to the pathogenesis of skeletal and cardiac muscles in LmnaH222P/H222P mutant mice: E. Wada, et al.; PLoS One
14, e0221512 (2019),
Abstract;
Full Text
ATP-Induced Increase in Intracellular Calcium Levels and Subsequent Activation of mTOR as Regulators of Skeletal Muscle Hypertrophy: N. Ito, et al.; Int. J. Mol. Sci.
19, 2804 (2018),
Abstract;
Full Text
Interleukin-1beta (IL-1β)-induced Notch ligand Jagged1 suppresses mitogenic action of IL-1β on human dystrophic myogenic cells: Y. Nagata Y, et al.; PLoS One
12, e0188821 (2017),
Application(s): Immunofluorescence,
Abstract;
Full Text
Sox2 expression in Schwann cells inhibits myelination in vivo and induces influx of macrophages to the nerve: S.L. Roberts, et al.; Development
144, 3114 (2017),
Abstract;
Full Text
Treatment with the anti-IL-6 receptor antibody attenuates muscular dystrophy via promoting skeletal muscle regeneration in dystrophin-/utrophin-deficient mice: E. Wada, et al.; Skelet. Muscle
7, 23 (2017),
Abstract;
Full Text
Treatment with the anti-IL-6 receptor antibody attenuates musculardystrophy via promoting skeletal muscle regeneration in dystrophin-/utrophin-deficient mice: E. Wada, et al.; Skelet. Muscle
7, 23 (2017),
Abstract;
Full Text
Enhancement of Satellite Cell Transplantation Efficiency by Leukemia Inhibitory Factor: N. Ito, et al.; J. Neuromuscul. Dis.
3, 201 (2016),
Abstract;
Full Text
Impaired regenerative capacity and lower revertant fibre expansion in dystrophin-deficient mdx muscles on DBA/2 background: M. Rodrigues, et al.; Sci. Rep.
6, 38371 (2016),
Abstract;
Full Text
YAP and TAZ control peripheral myelination and the expression of laminin receptors in Schwann cells: Y. Poitelon, et al.; Nat. Neurosci.
19, 879 (2016),
Abstract;
Full Text
AKAP6 inhibition impairs myoblast differentiation and muscle regeneration: Positive loop between AKAP6 and myogenin: S.W. Lee, et al.; Sci. Rep.
5, 16523 (2015),
Abstract;
Full Text
MicroRNA-26a induced by hypoxia targets HDAC6 in myogenic differentiation of embryonic stem cells: S.W. Lee, et al.; Nucleic Acids Res.
43, 2057 (2015),
Application(s): Immunofluorescence,
Abstract;
Full Text
Selective Retinoic Acid Receptor γ Agonists Promote Repair of Injured Skeletal Muscle in Mouse: A. Di Rocco, et al.; Am. J. Pathol.
185, 2495 (2015),
Abstract;
Full Text
Dystroglycan does not contribute significantly to kidney development or function, in health or after injury: G. Jarad, et al.; Am. J. Physiol. Renal Physiol.
300, F811 (2011),
Abstract;
Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells: Y. Mizuno, et al.; FASEB J.
24, 2245 (2010),
Abstract;
Maintenance of glomerular filtration barrier integrity requires laminin alpha5: S. Goldberg, et al.; J. Am. Soc. Nephrol.
21, 579 (2010),
Abstract;
Full Text
A single point mutation in the LN domain of LAMA2 causes muscular dystrophy and peripheral amyelination: BL. Patton, et al.; J. Cell Sci.
121, 1593 (2008),
Abstract;
Hematopoietic Contribution to Skeletal Muscle Regeneration in Acid Alpha-glucosidase Knockout Mice.: J. Mori, et al.; J. Histochem. Cytochem.
56, 811 (2008),
Abstract;
Lung development in laminin gamma2 deficiency: abnormal tracheal hemidesmosomes with normal branching morphogenesis and epithelial differentiation: N.M. Nguyen, et al.; Respir. Res.
7, 28 (2006),
Abstract;
Full Text
Laminin alpha2 is essential for odontoblast differentiation regulating dentin sialoprotein expression: K. Yuasa, et al.; J. Biol. Chem.
279, 10286 (2004),
Abstract;
Full Text
Laminins alpha2 and alpha4 in pancreatic acinar basement membranes are required for basal receptor localization: J.H. Miner, et al.; J. Histochem. Cytochem.
52, 153 (2004),
Abstract;
Full Text
Skeletal muscle engraftment potential of adult mouse skin side population cells: F. Montanaro, et al.; PNAS
100, 9336 (2003),
Abstract;
Full Text
Characterization of bone marrow laminins and identification of alpha5-containing laminins as adhesive proteins for multipotent hematopoietic FDCP-Mix cells: Y. Gu, et al.; Blood
93, 2533 (1999),
Abstract;
Full Text
Loss of the sarcoglycan complex and sarcospan leads to muscular dystrophy in beta-sarcoglycan-deficient mice: K. Araishi, et al.; Hum. Mol. Genet.
8, 1589 (1999),
Abstract;
Full Text
Mild congenital muscular dystrophy in two patients with an internally deleted laminin alpha2-chain: V. Allamand, et al.; Hum. Mol. Gen.
6, 747 (1997),
Abstract;
Full Text
Diagnosis of merosin (laminin-2) deficient congenital muscular dystrophy by skin biopsy: C.A. Sewry, et al.; Lancet
347, 582 (1996),
Abstract;
Expression of laminin isoforms in mouse myogenic cells in vitro and in vivo: F. Schuler & L.M. Sorokin; J. Cell Sci.
108, 3795 (1995),
Abstract;
Full Text
General Literature References
Physiological and pathological implications of laminins: from the gene to the protein: M.C. Paez, et al.; Autoimmunity
40, 83 (2007), (Review),
Abstract;
Laminin-induced signaling in tumor cells: V. Givant-Horwitz, et al.; Cancer Lett.
223, 1 (2005), (Review),
Abstract;
Laminin: the crux of basement membrane assembly: T. Sasaki, et al.; J. Cell Biol.
164, 959 (2004), (Review),
Abstract;
Full Text
Form and function: the laminin family of heterotrimers: H. Colognato & P.D. Yurchenko; Dev. Dyn.
218, 213 (2000), (Review),
Abstract;
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