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[pTyr705]STAT3 monoclonal antibody (9E12)

ALX-804-180-C100 100 µg 458.00 USD
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Product Details

Alternative Name:Signal transducer and activator of transcription 3
Immunogen:Synthetic peptide corresponding to a portion of STAT3 phosphorylated at Tyr705 conjugated to KLH.
UniProt ID:P40763
Species reactivity:Human, Mouse
Specificity:Recognizes the -AAPpYLKT-motif of activated STAT3 phosphorylated at Tyr705.
Crossreactivity:Does not cross-react with the non-phosphorylated form of STAT3 nor with unrelated tyrosine-phosphorylated proteins.
Applications:ELISA, ICC, IHC, IP, WB
Recommended Dilutions/Conditions:Western Blot (0.1µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Positive Control:Included. (Prod. No. ALX-840-025)
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STAT3 is activated by tyrosine phosphorylation at residue Y705 in cells treated with interleukin-6 or EGF. Activated STAT3 can bind to DNA either as homodimer or as heterodimer with STAT1.
Technical Info/Product Notes:Replacement for ADI-905-671
Regulatory Status:RUO - Research Use Only

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