| ALX-804-170-C100 | 100 µg | 498.00 USD |  |
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| Clone: | 14B3 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Phosphothreonine containing peptides conjugated to KLH. |
| Species reactivity: | Species independent |
| Specificity: | Recognizes a broad range of threonine-phosphorylated proteins. |
| Applications: | ELISA, IP, WB |
| Recommended Dilutions/Conditions: | ELISA (use at 0.05µg/ml) Immunoprecipitation (1-10µg per 106 pervanadate-treated A431 cells) Western Blot (1µg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20. Do not use milk/casein for blocking and dilution! Replace by BSA!) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Positive Control: | Included. (Prod. No. 840-012-1) |
| Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
| Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
| Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
| Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 1 week. |
| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Technical Info/Product Notes: | Replacement for ADI-905-807 |
| Regulatory Status: | RUO - Research Use Only |
Phosphothreonine detection set |
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| IP, WB | Print as PDF | ||||||
| ALX-850-024-KI01 | 1 Set | 540.00 USD | |
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