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Phosphothreonine monoclonal antibody (1E11)

ALX-804-168-C100 100 µg 690.00 USD
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Replaces Prod. #: BML-SA338

Product Details

Immunogen:Phosphothreonine containing peptides conjugated to KLH.
Species reactivity:Species independent
Specificity:Recognizes a broad range of threonine-phosphorylated proteins.
Applications:ELISA, IP, WB
Recommended Dilutions/Conditions:Western Blot (1µg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20. Do not use milk/casein for blocking and dilution! Replace by BSA!)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Positive Control:Included. (Prod. No. 840-012-1)
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 1 week.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Shipping:Blue Ice
Long Term Storage:-20°C
Technical Info/Product Notes:Replacement for ADI-905-808
Regulatory Status:RUO - Research Use Only

Product Literature References

Regulation of the ligand binding activity of the human very low density lipoprotein receptor by protein kinase C-dependent phosphorylation: R. Sakthivel, et al.; J. Biol. Chem. 276, 555 (2001), Abstract; Full Text
Insulin-mimetic signalling of synthetic phosphoinositolglycans in isolated rat adipocytes: W. Frick, et al.; Biochem. J. 336 (Pt 1), 163 (1998), Abstract; Full Text

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