Replaces Prod. #: BML-SA338
Product Details
| Clone: | 1E11 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Phosphothreonine containing peptides conjugated to KLH. |
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| Species reactivity: | Species independent
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| Specificity: | Recognizes a broad range of threonine-phosphorylated proteins. |
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| Applications: | ELISA, IP, WB
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| Recommended Dilutions/Conditions: | Western Blot (1µg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20. Do not use milk/casein for blocking and dilution! Replace by BSA!)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Positive Control: | Included. (Prod. No. 840-012-1) |
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| Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
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| Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
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| Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
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| Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 1 week. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Technical Info/Product Notes: | Replacement for ADI-905-808 |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Regulation of the ligand binding activity of the human very low density lipoprotein receptor by protein kinase C-dependent phosphorylation: R. Sakthivel, et al.; J. Biol. Chem.
276, 555 (2001),
Abstract;
Full Text
Insulin-mimetic signalling of synthetic phosphoinositolglycans in isolated rat adipocytes: W. Frick, et al.; Biochem. J.
336 (Pt 1), 163 (1998),
Abstract;
Full Text
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