Replaces Prod. #: BML-SA337
Product Details
| Clone: | 16B4 |
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| Host: | Mouse |
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| Isotype: | IgM |
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| Immunogen: | Phosphoserine containing peptides conjugated to KLH. |
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| Species reactivity: | Species independent
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| Specificity: | Recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, strongly interacting with pSK and pSP motifs, thus specifically recognizing substrates of MAP/SAP kinases and CDC kinases. Please note that phosphoserine detection by MAbs always depends on the surrounding aa sequence. |
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| Applications: | ELISA, IP, WB
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| Recommended Dilutions/Conditions: | ELISA (use at 0.05µg/ml)
Western Blot (1µg/ml HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20. Do not use milk/casein for blocking and dilution! Replace by BSA!)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Positive Control: | Included. (Prod. No. 840-012-1) |
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| Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
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| Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
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| Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
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| Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 1 week. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Technical Info/Product Notes: | Replacement for ADI-905-805 |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Protein phosphatase 2A regulates apoptosis in neutrophils by dephosphorylating both p38 MAPK and its substrate caspase 3: M. Alvarado-Kristensson and T. Andersson; J. Biol. Chem.
280, 6238 (2005),
Abstract;
Full Text
p38-MAPK signals survival by phosphorylation of caspase-8 and caspase-3 in human neutrophils: M. Alvarado-Kristensson, et al.; J. Exp. Med.
199, 449 (2004),
Abstract;
Full Text
Phosphorylation of Bax Ser184 by Akt regulates its activity and apoptosis in neutrophils: S.J. Gardai, et al.; J. Biol. Chem.
279, 21085 (2004),
Abstract;
Full Text
Kennedy’s disease. Phosphorylation of the polyglutamine-expanded form of androgen receptor regulates its cleavage by caspase-3 and enhances cell death: M.A. LaFevre-Bernt and L.M. Ellerby; J. Biol. Chem.
278, 34918 (2003),
Abstract;
Full Text
Towards a phosphoproteome map of Corynebacterium glutamicum: A.K. Bendt, et al.; Proteomics
3, 1637 (2003),
Abstract;
Glyceraldehyde-3-phosphate dehydrogenase is phosphorylated by protein kinase Ciota /lambda and plays a role in microtubule dynamics in the early secretory pathway: E.J. Tisdale; J. Biol. Chem.
277, 3334 (2002),
Abstract;
Full Text
Human phosphatidylinositol 4-kinase isoform PI4K92. Expression of the recombinant enzyme and determination of multiple phosphorylation sites: S. Suer, et al.; Eur. J. Biochem.
268, 2099 (2001),
Abstract;
Full Text
MID1, mutated in Opitz syndrome, encodes an ubiquitin ligase that targets phosphatase 2A for degradation: A. Trockenbacher, et al.; Nat. Genet.
29, 287 (2001),
Abstract;
Full Text
Insulin selectively stimulates nuclear phosphoinositide-specific phospholipase C (PI-PLC) beta1 activity through a mitogen-activated protein (MAP) kinase-dependent serine phosphorylation: A.M. Martelli, et al.; FEBS Lett.
486, 230 (2000),
Abstract;
The ternary complex factor Net contains two distinct elements that mediate different responses to MAP kinase signalling cascades: C. Ducret, et al.; Oncogene
19, 5063 (2000),
Abstract;
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