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United States 

Phosphoserine monoclonal antibody (4A9)

ALX-804-164-C100 100 µg 481.00 USD
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Replaces Prod. #: BML-SA334

Product Details

Immunogen:Phosphoserine containing peptides conjugated to KLH.
Species reactivity:Species independent
Specificity:Recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, interacting with phosphoserine surrounded by positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.). Please note that phosphoserine detection by MAbs always depends on the surrounding aa sequence.
Applications:ELISA, IP, WB
Recommended Dilutions/Conditions:Western Blot (1µg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20. Do not use milk/casein for blocking and dilution! Replace by BSA!)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Positive Control:Included. (Prod. No. 840-012-1)
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 1 week.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Shipping:Blue Ice
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only

Product Literature References

Coordination of membrane excitability through a GIRK1 signaling complex in the atria: E.N. Nikolov and T.T. Ivanova-Nikolova; J. Biol. Chem. 279, 23630 (2004), Abstract; Full Text
Towards a phosphoproteome map of Corynebacterium glutamicum: A.K. Bendt, et al.; Proteomics 3, 1637 (2003), Abstract;
Light regulation of protein phosphorylation in Blepharisma japonicum: H. Fabzak et al.; Acta Protozool. 40, 311 (2001),
The chloroplastic glutamine synthetase (GS-2) of tobacco is phosphorylated and associated with 14-3-3 proteins inside the chloroplast: J. Riedel, et al.; Planta 213, 396 (2001), Abstract;
Nitric oxide-sensitive guanylyl cyclase activity inhibition through cyclic GMP-dependent dephosphorylation: R. Ferrero, et al.; J. Neurochem. 75, 2029 (2000), Abstract;
Insulin signaling in the yeast Saccharomyces cerevisiae. 3. Induction of protein phosphorylation by human insulin: G. Muller, et al.; Biochemistry 37, 8705 (1998), Abstract;
Insulin-mimetic signalling of synthetic phosphoinositolglycans in isolated rat adipocytes: W. Frick, et al.; Biochem. J. 336 (Pt 1), 163 (1998), Abstract; Full Text
Advanced glycation endproducts stimulate the MAP-kinase pathway in tubulus cell line LLC-PK1: A. Simm, et al.; FEBS Lett. 410, 481 (1997), Abstract;
Characterization and purification of human corneodesmosin, an epidermal basic glycoprotein associated with corneocyte-specific modified desmosomes: M. Simon, et al.; J. Biol. Chem. 272, 31770 (1997), Abstract; Full Text

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