Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Phosphoserine monoclonal antibody (4A3)

ALX-804-163-C100 100 µg 501.00 USD
Do you need bulk/larger quantities?
Replaces Prod. #: BML-SA333

Product Details

Immunogen:Phosphoserine containing peptides conjugated to KLH.
Species reactivity:Species independent
Specificity:Recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, interacting with phosphoserine surrounded by positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.). Please note that phosphoserine detection by MAbs always depends on the surrounding aa sequence.
Applications:ELISA, IP, WB
Recommended Dilutions/Conditions:Western Blot (1µg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20. Do not use milk/casein for blocking and dilution! Replace by BSA!)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Positive Control:Included. (Prod. No. 840-012-1)
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 1 week.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Shipping:Blue Ice
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only

Product Literature References

Towards a phosphoproteome map of Corynebacterium glutamicum: A.K. Bendt et al.; Proteomics 3, 1637 (2003), Abstract;
The neuropeptide proctolin induces phosphorylation of a 30 kDa protein associated with the thin filament in crustacean muscle: B. Brustle, et al.; J. Exp. Biol. 204, 2627 (2001), Abstract; Full Text
Insulin signaling in the yeast Saccharomyces cerevisiae. 3. Induction of protein phosphorylation by human insulin: G. Muller, et al.; Biochemistry 37, 8705 (1998), Abstract;
Characterization and purification of human corneodesmosin, an epidermal basic glycoprotein associated with corneocyte-specific modified desmosomes: M. Simon, et al.; J. Biol. Chem. 272, 31770 (1997), Abstract; Full Text
Regulatory phosphorylation of the Na+/K+-ATPase from mammalian kidneys and Xenopus oocytes by protein kinases: L.A. Vasilets, et al.; Ann. N.Y. Acad. Sci. 834, 585 (1997), Abstract;

Related Products

Phosphoserine detection set 

IP, WB | Print as PDF
ALX-850-023-KI01 1 Set 907.00 USD
Do you need bulk/larger quantities?

Product Toolbox


Certificate of Analysis


By target:
By biological activity:
Phosphoprotein Monoclonal antibody
By catalog section:


Technical Service
Customer Service