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BAFF (mouse) monoclonal antibody (1C9)

ALX-804-157-C100 100 µg 441.00 USD
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Product Details

Alternative Name:B cell-activating factor, BLyS, TALL-1, THANK, zTNF4, TNFSF 13B/20, CD257
Immunogen:Rat RBL cells expressing mouse BAFF.
UniProt ID:Q9WU72
Source:Purified from hybridoma tissue culture supernatant.
Species reactivity:Mouse
Applications:ELISA, Flow Cytometry
Recommended Dilutions/Conditions:ELISA (detection)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
BAFF (mouse) monoclonal antibody (1C9) Flow Cytometry
Method: HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 105) were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1µg/ml of MAb to BAFF (mouse) (1C9). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG (Prod. No. ALX-211-053) was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry."
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BAFF (mouse) monoclonal antibody (1C9) Flow Cytometry

Product Literature References

TACI, unlike BAFF-R, is solely activated by oligomeric BAFF and APRIL to support survival of activated B cells and plasmablasts: C. Bossen, et al.; Blood 111, 1004 (2008), Abstract;
TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma: M. Batten, et al.; J. Immunol. 172, 812 (2004), Abstract;

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