Product Specification
| Alternative Name: | TAJ, TNFRSF 19 |
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| Clone: | Giu.Li |
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| Host: | Rat |
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| Isotype: | IgG |
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| Immunogen: | Recombinant human Troy cysteine-rich domain (aa 1-168). |
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| UniProt ID: | Q9NS68 |
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| Source: | Purified from concentrated hybridoma tissue culture supernatant. |
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| Species reactivity: | Human
|
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| Applications: | Flow Cytometry
|
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored as recommended. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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Figure: HEK 293T cells were mock transfected (thin line) or transfected with an expression coding for the extracellular domain of human Troy (thick line).
Method: Cells (5x10
5) were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1µg/ml of monoclonal antibody to Troy (Giu.Li). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG (Prod. No.
ALX-211-053) was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry."
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General Literature References
TRAF6-deficient mice display hypohidrotic ectodermal dysplasia: A. Naito, et al.; PNAS
99, 8766 (2002),
Abstract;
Full Text
TAJ, a novel member of the tumor necrosis factor receptor family, activates the c-Jun N-terminal kinase pathway and mediates caspase-independent cell death: M.T. Eby, et al.; J. Biol. Chem.
275, 15336 (2000),
Abstract;
Full Text
TROY, a newly identified member of the tumor necrosis factor receptor superfamily, exhibits a homology with Edar and is expressed in embryonic skin and hair follicles: T. Kojima, et al.; J. Biol. Chem.
275, 20742 (2000),
Abstract;
Full Text
