Figure 1: MAb to APRIL (Sacha-1) (Prod. No.
ALX-804-141) detects membrane-bound human APRIL by FACS.
Method: HEK 293T cells (5x10
5) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of a non-cleavable mouse APRIL (thick line). Cells were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of MAb to APRIL (Sacha-1) (Prod. No.
ALX-804-141). After washing in FACS buffer, PE-conjugated antibody to rat IgG (Prod. No.
ALX-211-053) was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry."