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APRIL monoclonal antibody (Sacha-1)



ALX-804-141-C100	100 µg	338.00 USD
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Product Specification

Alternative Name:	TNFSF 13, A-Proliferation-inducing ligand, CD256

Clone:	Sacha-1

Host:	Rat

Isotype:	IgG2a

Immunogen:	Recombinant human APRIL (aa 105-250) fused to human ACRP30headless (aa 16-108) (Prod. No. ALX-522-035).

UniProt ID:	O75888

Source:	Purified from concentrated hybridoma tissue culture supernatant.

Species reactivity:	Human, Mouse

Specificity:	Excellent for mouse April; also stains human APRIL.

Applications:	Flow Cytometry, ICC

Formulation:	Liquid. In PBS containing 0.02% sodium azide.

Use/Stability:	Stable for at least 6 months after receipt when stored at +4°C undiluted.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Blue Ice Not Frozen

Long Term Storage:	+4°C

Regulatory Status:	RUO - Research Use Only

APRIL monoclonal antibody (Sacha-1) Flow Cytometry

Figure 1: MAb to APRIL (Sacha-1) (Prod. No. ALX-804-141) detects membrane-bound human APRIL by FACS.Method: HEK 293T cells (5x10⁵) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of a non-cleavable mouse APRIL (thick line). Cells were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of MAb to APRIL (Sacha-1) (Prod. No. ALX-804-141). After washing in FACS buffer, PE-conjugated antibody to rat IgG (Prod. No. ALX-211-053) was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry."

APRIL monoclonal antibody (Sacha-1) image

Figure 2: Immunostaining of HEK-293T cells transfected with a murine APRIL expression plasmid (left panels), or mock transfected (right panel).Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone. Slides were blocked with IgG, and incubated for 1 hour with 5µg/ml Sacha-1 or control murine IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After washes wish PBS, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.

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