Product Details
| Clone: | Kimmy-1 |
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| Host: | Rat |
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| Isotype: | IgG2a |
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| Immunogen: | Recombinant mouse Tollip. |
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| UniProt ID: | Q9QZ06 |
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| Source: | Purified from hybridoma tissue culture supernatant. |
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| Species reactivity: | Human, Mouse
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| Applications: | IP, WB
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| Recommended Dilutions/Conditions: | Western Blot (5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C undiluted. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Detection of endogenous tollip in murine EL-4. Optimal dilution of Kimmy-1 is between 1-5µg/ml. Extracts should be made from approximately 10
6 -10
7 cells.
Method: RIPA buffer extracts from 1.5x10
6 EL-4 cells were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with Kimmy-1 at a dilution of 5µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG (
ALX-211-052) and a chemiluminescence detection system."
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Product Literature References
Tollip interaction with STAT3: a novel mechanism to regulate human airway epithelial responses to type 2 cytokines: N. Schaunaman, et al.; Respir. Res.
23, 31 (2022),
Abstract;
Threonine 66 in the death domain of IRAK-1 is critical for interaction with signaling molecules but is not a target site for autophosphorylation: D. Neumann, et al.; J. Leukoc. Biol.
84, 807 (2008),
Abstract;
Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor: K. Burns, et al.; Nat. Cell Biol.
2, 346 (2000),
Abstract;
