Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

BAFF monoclonal antibody (Buffy-2)

ALX-804-131-C100 100 µg 443.00 USD
Do you need bulk/larger quantities?

Product Details

Alternative Name:B cell-activating factor, BLyS, TALL-1, THANK, zTNF4, TNFSF 13B/20, CD257
Immunogen:Recombinant human soluble BAFF (aa 83-285) (Prod. No. ALX-522-025).
UniProt ID:Q9Y275
Source:Purified from ascites.
Species reactivity:Human
Specificity:Recognizes membrane-bound and soluble BAFF.
Crossreactivity:Weakly cross-reacts with mouse BAFF.
Applications:Flow Cytometry, IHC (FS), IHC (PS), WB
Purity Detail:Purified by IgM purification HP column.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Use/Stability:Stable for at least 6 months after receipt when stored as recommended.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Regulatory Status:RUO - Research Use Only
BAFF monoclonal antibody (Buffy-2) Schematic structure of immunogen
Schematic structure of rhsBAFF (aa. 83-285). MAb to BAFF (Buffy-2) recognizes the TNF-homology domain.
BAFF monoclonal antibody (Buffy-2) Immunohistochemistry
Figure: Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using Buffy-2.
Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.
Please mouse over
BAFF monoclonal antibody (Buffy-2) Schematic structure of immunogen BAFF monoclonal antibody (Buffy-2) Immunohistochemistry

Product Literature References

APRIL binding to BCMA activates a JNK2-FOXO3-GADD45 pathway and induces a G2/M cell growth arrest in liver cells: G. Notas, et al.; J. Immunol. 189, 4748 (2012), Abstract;
Detection of the TNFSF members BAFF, APRIL, TWEAK and their receptors in normal kidney and renal cell carcinomas: V. Pelekanou, et al.; Anal. Cell. Pathol. (Amst.) 34, 49 (2011), Abstract;
Adipocytes as immune cells: differential expression of TWEAK, BAFF, and APRIL and their receptors (Fn14, BAFF-R, TACI, and BCMA) at different stages of normal and pathological adipose tissue development: V.I. Alexaki, et al.; J. Immunol. 183, 5948 (2009), Abstract;
Systemic activation of the immune system induces aberrant BAFF and APRIL expression in B cells in patients with systemic lupus erythematosus: V.T. Chu, et al.; Arthritis Rheum. 60, 2083 (2009), Application(s): Flow Cytometry, Abstract;
Expression of TNF-superfamily members BAFF and APRIL in breast cancer: Immunohistochemical study in 52 invasive ductal breast carcinomas: V. Pelekanou, et al.; BMC Cancer 8, 76 (2008), Abstract; Full Text
TACI, unlike BAFF-R, is solely activated by oligomeric BAFF and APRIL to support survival of activated B cells and plasmablasts: C. Bossen, et al.; Blood 111, 1004 (2008), Application(s): Western Blot , Abstract;
In vitro and in vivo activation induces BAFF and APRIL expression in B cells: V.T. Chu, et al.; J. Immunol. 179, 5947 (2007), Application(s): Flow Cytometry of B Cells, Abstract; Full Text
Association between circulating levels of the novel TNF family members APRIL and BAFF and lymphoid organization in primary Sjogren’s syndrome: M.V. Jonsson, et al.; J. Clin. Immunol. 25, 189 (2005), Abstract;
Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt’s lymphoma: C.A. Ogden, et al.; J. Immunol. 174, 3015 (2005), Abstract; Full Text
BAFF production by antigen-presenting cells provides T cell co-stimulation: B. Huard, et al.; Int. Immunol. 16, 467 (2004), Abstract;
Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival: A. J. Novak, et al.; Blood 103, 689 (2004), Abstract;
Expression of BLyS and its Receptors in B-Cell Non-Hodgkin Lymphoma: Correlation with Disease Activity and Patient Outcome: A.J. Novak, et al.; Blood 104, 2247 (2004), Abstract;
Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL: B. He, et al.; J. Immunol. 172, 3268 (2004), Abstract; Full Text
Association of BAFF/BLyS overexpression and altered B cell differentiation with Sjogren's syndrome: J. Groom, et al.; J. Clin. Invest. 109, 59 (2002), Abstract; Full Text

General Literature References

Severe B cell hyperplasia and autoimmune disease in TALL-1 transgenic mice: S.D. Khare, et al.; PNAS 97, 3370 (2000), Abstract; Full Text
BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth: P. Schneider, et al.; J. Exp. Med. 189, 1747 (1999), Abstract; Full Text
BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator: P.A. Moore, et al.; Science 285, 260 (1999), Abstract;
Identification and characterization of a novel cytokine, THANK, a TNF homologue that activates apoptosis, nuclear factor-kB, and c-Jun NH2-terminal kinase: A. Mukhopadhyay, et al.; J. Biol. Chem. 274, 15978 (1999), Abstract; Full Text
Mice transgenic for BAFF develop lymphocytic disorders along with autoimmune manifestations: F. Mackay, et al.; J. Exp. Med. 190, 1697 (1999), Abstract; Full Text
TALL-1 is a novel member of the TNF family that is down-regulated by mitogens: H.B. Shu, et al.; J. Leukoc. Biol. 65, 680 (1999), Abstract;

Related Products

BAFF (soluble) (human), (recombinant) 

Produced in E. coli. The extracellular domain of human BAFF (aa 83-285) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG®-tag., ≥95% (SDS-PAGE), ELISA | Print as PDF
ALX-522-025-C010 10 µg 563.00 USD
Do you need bulk/larger quantities?

Product Toolbox


Certificate of Analysis


By target:
By biological activity:
BAFF Monoclonal antibody
By catalog section:


Technical Service
Customer Service