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FasL (human) monoclonal antibody (Mike-2)

ALX-804-125-C100 100 µg 358.00 USD
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Product Details

Alternative Name:CD95L, APO-1L, CD178, TNFSF 6
Immunogen:Recombinant human soluble FasL (CD95L; APO-1L; CD178) (aa 103-281).
UniProt ID:P48023
Source:Purified from hybridoma tissue culture supernatant.
Species reactivity:Human
Specificity:Recognizes only the membrane-bound active (non-cleaved) FasL and not the inactive soluble FasL.
Applications:Flow Cytometry, WB
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Use/Stability:Stable for at least 6 months after receipt when stored at +4°C undiluted.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Regulatory Status:RUO - Research Use Only
FasL (human) monoclonal antibody (Mike-2) Schematic structure of immunogen
Schematic structure of Human FasL (recombinant, aa 103-281). The antibody recognizes an epitope within aa 103-126.
FasL (human) monoclonal antibody (Mike-2) image
Figure: Detection of membrane-bound FasL in HEK 293T cells transfected with a human FasL expression plasmid (right lane) compared to untransfected cells (left lane). Arrows indicate FasL and glycosylated forms of FasL.Method: Cell extracts from FasL transfected cells (5x104) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-FasL Mike-2 antibody at 1 µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG (Prod. No. ALX-211-052) and a chemiluminescence detection system."
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FasL (human) monoclonal antibody (Mike-2) Schematic structure of immunogen FasL (human) monoclonal antibody (Mike-2) image

Product Literature References

Constitutive Expression of FasL in Thyrocytes: T.A. Stokes, et al.; Science 279, 2015a (1998), Full Text

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