Product Specification
| Alternative Name: | CD95L, APO-1L, CD178, TNFSF 6 |
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| Clone: | Mike-2 |
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| Host: | Rat |
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| Isotype: | IgG2a |
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| Immunogen: | Recombinant human soluble FasL (CD95L; APO-1L; CD178) (aa 103-281). |
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| UniProt ID: | P48023 |
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| Source: | Purified from hybridoma tissue culture supernatant. |
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| Species reactivity: | Human
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| Specificity: | Recognizes only the membrane-bound active (non-cleaved) FasL and not the inactive soluble FasL. |
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| Applications: | Flow Cytometry, WB
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at +4°C undiluted. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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Schematic structure of Human FasL (recombinant, aa 103-281). The antibody recognizes an epitope within aa 103-126.
Figure: Detection of membrane-bound FasL in HEK 293T cells transfected with a human FasL expression plasmid (right lane) compared to untransfected cells (left lane). Arrows indicate FasL and glycosylated forms of FasL.
Method: Cell extracts from FasL transfected cells (5x10
4) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-FasL Mike-2 antibody at 1 µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG (Prod. No.
ALX-211-052) and a chemiluminescence detection system."
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Product Literature References
Constitutive Expression of FasL in Thyrocytes: T.A. Stokes, et al.; Science
279, 2015a (1998),
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