Product Specification
| Alternative Name: | CD95L, APO-1L, CD178, TNFSF 6 |
| |
| Clone: | Mike-2 |
| |
| Host: | Rat |
| |
| Isotype: | IgG2a |
| |
| Immunogen: | Recombinant human soluble FasL (CD95L; APO-1L; CD178) (aa 103-281). |
| |
| UniProt ID: | P48023 |
| |
| Source: | Purified from hybridoma tissue culture supernatant. |
| |
| Species reactivity: | Human
|
| |
| Specificity: | Recognizes only the membrane-bound active (non-cleaved) FasL and not the inactive soluble FasL. |
| |
| Applications: | Flow Cytometry, WB
|
| |
| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
| |
| Use/Stability: | Stable for at least 6 months after receipt when stored at +4°C undiluted. |
| |
| Handling: | Avoid freeze/thaw cycles. |
| |
| Shipping: | Blue Ice Not Frozen |
| |
| Long Term Storage: | +4°C |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Schematic structure of Human FasL (recombinant, aa 103-281). The antibody recognizes an epitope within aa 103-126.
Figure: Detection of membrane-bound FasL in HEK 293T cells transfected with a human FasL expression plasmid (right lane) compared to untransfected cells (left lane). Arrows indicate FasL and glycosylated forms of FasL.
Method: Cell extracts from FasL transfected cells (5x10
4) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-FasL Mike-2 antibody at 1 µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG (Prod. No.
ALX-211-052) and a chemiluminescence detection system."
Please mouse over
Product Literature References
Constitutive Expression of FasL in Thyrocytes: T.A. Stokes, et al.; Science
279, 2015a (1998),
Full Text
