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Cytochrome c (apo) monoclonal antibody (SJL2-4(apo))

ALX-804-122-C100 100 µg 264.00 USD
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Product Specification

Alternative Name:Cyt c (apo)
Immunogen:Horse cytochrome c.
UniProt ID:P00004
Species reactivity:Human, Mouse, Rat
Goat, Monkey, Sheep
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Use/Stability:Stable for at least 6 months after receipt when stored at +4°C undiluted.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Regulatory Status:RUO - Research Use Only
Cytochrome c (apo) monoclonal antibody (SJL2-4(apo)) Western blot
Detection of cytochrome c in human cells. Cytoplasmic fraction (1), nuclear fraction (2). Cytochrome c migrates as a ~13kDa species. Method: Cell extracts from 293T cells (2x106) were separated into cytoplasmic and nuclear fraction, resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-cytochrome c antibody SJL2-4 (apo) at 1µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.
Cytochrome c (apo) monoclonal antibody (SJL2-4(apo)) Schematic structure of immunogen
Schematic structure of Horse cytochrome c.
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Cytochrome c (apo) monoclonal antibody (SJL2-4(apo)) Western blot Cytochrome c (apo) monoclonal antibody (SJL2-4(apo)) Schematic structure of immunogen

Product Literature References

Differential effects of two anti-apo-cytochrome c-specific monoclonal antibodies on the function of apo-cytochrome c-specific murine T cell clones: G. Corradin, et al.; J. Immunol. 133, 2915 (1984), Abstract;

General Literature References

Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3: H. Zou, et al.; Cell 90, 405 (1997), Abstract;
Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell 91, 479 (1997), Abstract;
Cytochrome c: can't live with it-can't live without it: J.C. Reed; Cell 91, 559 (1997), Abstract;
Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked: J. Yang, et al.; Science 275, 1129 (1997), Abstract;

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