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Granzyme B (human) monoclonal antibody (B18.1)

ALX-804-121-C100 100 µg Purified 353.00 USD
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Product Details

Immunogen:Recombinant human granzyme B (aa 7-227).
UniProt ID:P10144
Source:Purified from mouse ascites.
Species reactivity:Human
Applications:Flow Cytometry, ICC, IHC (FS), IHC (PS), IP, WB
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Use/Stability:Stable for at least 6 months after receipt when stored at +4°C undiluted.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
Granzyme B (human) monoclonal antibody (B18.1) Schematic structure of immunogen
Schematic structure of human granzyme B (recombinant, aa. 7-227).
Granzyme B (human) monoclonal antibody (B18.1) image
Figure: Detection of recombinant granzyme B. Granzyme B migrates as a 27 kDa species.
Method: Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.
Granzyme B (human) monoclonal antibody (B18.1) image
Figure: Detection of granzyme B in a human CD8+ T cell clone.
Method: Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at room temperature (RT) with 20 µg/ml of B18.4 antibody in PBS, 0.1% BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.
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Granzyme B (human) monoclonal antibody (B18.1) Schematic structure of immunogen Granzyme B (human) monoclonal antibody (B18.1) image Granzyme B (human) monoclonal antibody (B18.1) image

Product Literature References

Granzyme-mediated cytotoxicity does not involve the mannose 6-phosphate receptors on target cells: R. Dressel, et al.; J. Biol. Chem. 279, 20200 (2004), Abstract; Full Text
Acquisition of granzyme B and Fas ligand proteins by human keratinocytes contributes to epidermal cell defense: C. Berthou, et al.; J. Immunol. 159, 5293 (1997), Abstract;
Expansion of a peripheral blood perforin+ CD8+ T-cell subset in long- term surviving lung transplanted patients: C. Berthou, et al.; Transplant. Proc. 28, 1964 (1996), Abstract;
Granzyme B and perforin lytic proteins are expressed in CD34+ peripheral blood progenitor cells mobilized by chemotherapy and granulocyte colony-stimulating factor: C. Berthou, et al.; Blood 86, 3500 (1995), Abstract;
Granzyme B and perforin can be used as predictive markers of acute rejection in heart transplantation: S. Legros-Maida, et al.; Eur. J. Immunol. 24, 229 (1994), Abstract;
Perforin and granzyme B expression is associated with severe acute rejection. Evidence for in situ localization in alveolar lymphocytes of lung-transplanted patients: M.V. Clement, et al.; Transplantation 57, 322 (1994), Abstract;

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