Product Details
| Clone: | B18.1 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant human granzyme B (aa 7-227). |
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| UniProt ID: | P10144 |
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| Source: | Purified from mouse ascites. |
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| Species reactivity: | Human
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| Applications: | Flow Cytometry, ICC, IHC (FS), IHC (PS), IP, WB
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at +4°C undiluted. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Schematic structure of human granzyme B (recombinant, aa. 7-227).
Figure: Detection of recombinant granzyme B. Granzyme B migrates as a 27 kDa species.
Method: Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.
Figure: Detection of granzyme B in a human CD8+ T cell clone.
Method: Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at room temperature (RT) with 20 µg/ml of B18.4 antibody in PBS, 0.1% BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.
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Product Literature References
Granzyme-mediated cytotoxicity does not involve the mannose 6-phosphate receptors on target cells: R. Dressel, et al.; J. Biol. Chem.
279, 20200 (2004),
Abstract;
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Acquisition of granzyme B and Fas ligand proteins by human keratinocytes contributes to epidermal cell defense: C. Berthou, et al.; J. Immunol.
159, 5293 (1997),
Abstract;
Expansion of a peripheral blood perforin+ CD8+ T-cell subset in long- term surviving lung transplanted patients: C. Berthou, et al.; Transplant. Proc.
28, 1964 (1996),
Abstract;
Granzyme B and perforin lytic proteins are expressed in CD34+ peripheral blood progenitor cells mobilized by chemotherapy and granulocyte colony-stimulating factor: C. Berthou, et al.; Blood
86, 3500 (1995),
Abstract;
Granzyme B and perforin can be used as predictive markers of acute rejection in heart transplantation: S. Legros-Maida, et al.; Eur. J. Immunol.
24, 229 (1994),
Abstract;
Perforin and granzyme B expression is associated with severe acute rejection. Evidence for in situ localization in alveolar lymphocytes of lung-transplanted patients: M.V. Clement, et al.; Transplantation
57, 322 (1994),
Abstract;
