Product Details
Alternative Name: | Glc-NAc (O-linked) |
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Clone: | RL2 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Pore complex-lamina fraction purified from rat liver nuclear envelopes. |
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Species reactivity: | Human, Mouse, Rat
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Specificity: | By Western blot, ALX-804-111 recognizes up to eight different proteins from the nuclear pore complex (NPC) of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa as well as other O-linked glycoproteins outside of the NPC. |
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Applications: | ICC, IHC (PS), IP, WB
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Recommended Dilutions/Conditions: | Immunocytochemistry (1:100) Immunohistochemistry (paraffin sections, 1:200) Western Blot (1:1,000). Do not use with diluents containing glycosylated proteins Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects bands of ~210, ~180, ~145, ~100, ~63, ~58, ~54 and ~45kDa by Western blot. |
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Formulation: | Liquid. In PBS containing 0.05% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1: G. Majumdar, et al.; Diabetes
53, 3184 (2004),
Abstract;
Full Text
Monoclonal antibodies identify a group of nuclear pore complex glycoproteins: C.M. Snow, et al.; J. Cell. Biol.
104, 1143 (1987),
Abstract;