Product Details
Clone: | 15D9 |
|
Host: | Mouse |
|
Isotype: | IgG1 |
|
Immunogen: | Recombinant human α-catenin. |
|
UniProt ID: | P35221 (α1), P26232 (α2), Q9UI47 (α3) |
|
Species reactivity: | Human, Mouse, Rat Dog
|
|
Crossreactivity: | Does not cross-react with β-catenins. |
|
Applications: | ICC, IP, WB
|
|
Recommended Dilutions/Conditions: | Immunocytochemistry (0.1-1µg/ml; formaldehyde fixation is tolerated) Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
|
Positive Control: | Included. (Prod. No. ALX-840-026) |
|
Purity Detail: | Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography. |
|
Formulation: | Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose. |
|
Reconstitution: | Reconstitute with 1ml distilled water (15 min., RT). |
|
Use/Stability: | Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months. |
|
Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
|
Shipping: | Blue Ice |
|
Long Term Storage: | -20°C |
|
Regulatory Status: | RUO - Research Use Only |
|
Product Literature References
A simple elution strategy for biotinylated proteins bound to streptavidin conjugated beads using excess biotin and heat: J. S. Cheah, et al.; Biochem. Biophys. Res. Commun.
493, 1522 (2017),
Application(s): Western Blot on MDCK cells,
Abstract;
Control of cell-cell forces and collective cell dynamics by the intercellular adhesome: E. Bazellières, et al.; Nat. Cell Biol.
17, 409 (2016),
Abstract;