| ALX-804-095-R100 | 100 µl | 753.00 USD |  |
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| Alternative Name: | TGN38 |
| Clone: | 2F7.1 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide corresponding to aa 1-17 (L1PSASKPNNTS SENNPP17) of N-terminal rat TGN38 (trans-Golgi network 38). |
| UniProt ID: | P19814 |
| Species reactivity: | Human, Rat Monkey |
| Specificity: | Recognizes the luminal domain of TGN38. |
| Applications: | Flow Cytometry, ICC, WB |
| Recommended Dilutions/Conditions: | Immunocytochemistry (1:1,000) Western Blot (1:500) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Detects a band of ~38kDa by Western blot, representing deglycosylated TGN38. Under normal Western blot conditions detects a band of ~85-95kDa depending on cell type. |
| Formulation: | Liquid. Ascites diluted in PBS containing 0.05% sodium azide. |
| Handling: | Avoid freeze/thaw cycles. |
| Shipping: | Shipped on Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | The trans-Golgi network (TGN) is part of the secretory pathway of eukaryotic cells which is distinct from the Golgi stack.The TGN is important in the later stages of protein secretion where it seems to play a key role in the sorting and targeting of secreted proteins to the correct destination. Brefeldin A (Prod. No. BML-G405) causes the contents of the Golgi stack to be redistributed into the endoplasmatic reticulum while the TGN collapse upon the microtubule organizing center. This makes immunofluorescence detection of TGN38, following Brefeldin A treatment, a useful tool for the discrimination of the TGN from the Golgi stack. |
| Regulatory Status: | RUO - Research Use Only |
Brefeldin A |
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| GEF inhibitor | ||||||
| 20350-15-6, Isolated from Eupenicillium brefeldianum., ≥98% (HPLC) | Print as PDF | ||||||
| BML-G405-0005 | 5 mg | 117.00 USD | |
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| BML-G405-0025 | 25 mg | 416.00 USD | |
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| BML-G405-0100 | 100 mg | 1,198.00 USD | |
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