Product Details
Alternative Name: | Heat shock protein 90 |
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Clone: | 3G3 |
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Host: | Mouse |
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Isotype: | IgM |
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Immunogen: | Purified mouse HSP90 (heat shock protein 90). |
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UniProt ID: | P07901 (HSP90α), P11499 (HSP90β) |
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Species reactivity: | Human, Mouse, Rat Chicken, Rainbow trout
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Applications: | IF, IHC, IP, WB
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Recommended Dilutions/Conditions: | Immunofluorescence (1:200) Immunohistochemistry (1:200) Western Blot (1:500) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Ammonium sulfate/ascites suspension. |
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Formulation: | Liquid. Contains no preservatives. |
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Use/Stability: | Before use, centrifuge suspension (4’000 x g for 15 min.) and discard supernatant. Resuspend pellet in 200µl PBS, pH 7.2. If desired, add 0.05% sodium azide. Stable for 6 months after PBS resuspension.Removal of residual ammonium sulfate is not usually necessary for IP procedure; for other uses it may be removed by dialysis or equivalent method. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Evidence that the 90-kDa heat shock protein (HSP90) exists in cytosol in heteromeric complexes containing HSP70 and three other proteins with Mr of 63,000, 56,000, and 50,000: G.H. Perdew; J. Biol. Chem.
266, 6708 (1991),
Abstract;
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