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mtHSP70 monoclonal antibody (JG1)

ALX-804-077-R100 100 µl 722.00 USD
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Product Details

Alternative Name:Mortalin, Heat shock protein 70 (mitochondrial), GRP75
Immunogen:Synthetic peptide corresponding to C-terminal aa 615-633 (G615SGSSGTGEQKEDQKEEKQ633) of mouse mtHSP70 (mitochondrial heat shock protein 70).
UniProt ID:P38647
Species reactivity:Human, Mouse
Dog, Monkey
Applications:ICC, IP, WB
Recommended Dilutions/Conditions:Immunocytochemistry (1:50)
Western Blot (1:500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~75kDa by Western blot.
Purity Detail:Protein A purified. 
Formulation:Liquid. In PBS with 1 mg/mL BSA containing 0.05% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
mtHSP70 monoclonal antibody (JG1) ICC
Figure: Immunolocalization of mtHSP70 in human fibroblasts using MAb to to mtHSP70 (JG1) (Prod. No. ALX-804-077)."
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mtHSP70 monoclonal antibody (JG1) ICC

Product Literature References

Neuralized-like protein 4 (NEURL4) mediates ADP-ribosylation of mitochondrial proteins: M.D. Cardamone, et al.; J. Cell Biol. 221, e202101021 (2022), Abstract;
Ozone Activates the Nrf2 Pathway and Improves Preservation of Explanted Adipose Tissue In Vitro: B. Cisterna, et al.; Antioxidants (Basel) 9, 989 (2020), Abstract; Full Text
HDAC6 maintains mitochondrial connectivity under hypoxic stress by suppressing MARCH5/MITOL dependent MFN2 degradation: H.J. Kim, et al.; Biochem. Biophys. Res. Commun. 464, 1235 (2015), Application(s): Immunostaining, Abstract;
Characterization of stress response in human retinal epithelial cells: V. Giansanti, et al.; J. Cell. Mol. Med. 17, 103 (2013), Application(s): ICC on human adult retinal pigmented epithelial (ARPE-19) cells, Abstract; Full Text
Reversible interactions between smooth domains of the endoplasmic reticulum and mitochondria are regulated by physiological cytosolic Ca2+ levels: J.G. Goetz, et al.; J. Cell Sci. 120, 3553 (2007), Abstract; Full Text
Cytochrome c oxidase subassemblies in fibroblast cultures from patients carrying mutations in COX10, SCO1 or SURF1: S.L. Williams, et al.; J. Biol. Chem. 279, 7462 (2004), Abstract;
The life span determinant p66Shc localizes to mitochondria where it associates with mitochondrial heat shock protein 70 and regulates trans-membrane potential: F. Orsini, et al.; J. Biol. Chem. 279, 25689 (2004), Abstract;
Generation and characterization of monoclonal antibodies specific for members of the mammalian 70-kDa heat shock protein family: J.M. Green, et al.; Hybridoma 14, 347 (1995), Abstract;

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