| ALX-804-074-R250 | 250 µl | 743.00 USD |  |
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| Alternative Name: | Heat shock protein 70 (universal) |
| Clone: | 5A5 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Recombinant human HSP70 (heat shock protein 70). |
| UniProt ID: | P0DMV8 (HSPA1A), P0DMV9 (HSPA1B) |
| GenBank ID: | M11717 |
| Gene/Protein Identifier: | NP_005336.3 (RefSeq), NM_005345 (RefSeq), 3303 (Entrez GeneID), 140550 (OMIM) |
| Species reactivity: | Human, Mouse Avian, Drosophila, Fish, Flatworm, Frog, Yeast |
| Specificity: | Recognizes several members of the HSP70 gene family, including HSP70, HSC70, GRP78, and following heat shock, HSP72 in a broad range of species. |
| Crossreactivity: | HSP70, HSC70, GRP78, HSP72 (following heat shock) |
| Applications: | ICC, IF, IHC (FS), IP, WB Gel Shift |
| Recommended Dilutions/Conditions: | Immunocytochemistry (1:100) Immunofluorescence (1:50-100) Immunohistochemistry (frozen sections, 1:100-200) Immunoprecipitation (2µg) Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Detects bands from ~70 to ~78kDa by Western blot, representing different members of the HSP70 family. 2-D-gel electrophoresis is required to resolve the heat induced form of these proteins from their constitutively expressed counterparts. |
| Formulation: | Liquid. Ascites diluted in PBS containing 0.05% sodium azide. |
| Handling: | Avoid freeze/thaw cycles. |
| Shipping: | Shipped on Blue Ice |
| Long Term Storage: | -20°C |
| Regulatory Status: | RUO - Research Use Only |
