Product Details
| Alternative Name: | Heat shock cognate protein 70 |
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| Clone: | 13D3 |
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| Host: | Mouse |
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| Isotype: | IgM |
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| Immunogen: | Mouse spermatogenic cell protein. |
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| UniProt ID: | P63017 |
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| Species reactivity: | Human, Mouse, Rat
Cat, Monkey
|
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| Crossreactivity: | Slightly cross-reacts with HSP70. |
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| Applications: | IF, IHC, IP, WB
|
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| Recommended Dilutions/Conditions: | Immunofluorescence (1:20-1:200)
Immunohistochemistry (frozen sections, 1:20-1:200)
Immunoprecipitation (2 µl)
Western Blot (1:1,000-1:5,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~70kDa by Western blot. |
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| Formulation: | Liquid. Ascites diluted in PBS containing 0.05% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Stimulation of mTOR-independent autophagy and mitophagy by rilmenidine exacerbates the phenotype of transgenic TDP-43 mice: N.D. Perera, et al.; Neurobiol. Dis.
154, 105359 (2021),
Abstract;
Replication study: Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET: J. Kim, et al.; Elife
7, e39944 (2018),
Abstract;
Full Text
The blue light-induced interaction of cryptochrome 1 with COP1 requires SPA proteins during Arabidopsis light signaling: X. Holtkotte, et al.; PLoS Genet.
13, e1007044 (2017),
Abstract;
Full Text
Registered report: Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET: J. Lesnik, et al.; eLife
5, e07383 (2016),
Application(s): Western blot,
Abstract;
E3 ligase CHIP and Hsc70 regulate Kv1.5 protein expression and function in mammalian cells: P. Li, et al. ; J. Mol Cell. Cardiol. 86, 138 (2015), Application(s): Kv1.5 Degradation ,
Germline duplication of ATG2B and GSKIP predisposes to familial myeloid malignancies: J. Saliba, et al. ; Nat. Genet.
47, 1131 (2015),
Application(s): Immunoblotting,
Abstract;
Heat-shock cognate protein (hsc71) and related proteins in mouse spermatogenic cells: M. Maekawa, et al.; Biol. Reprod.
40, 843 (1989),
Abstract;
