Online Purchasing Account You are logged on as Guest. Login Register a New Account Shopping cart (Empty)

β-Catenin (exon 3) monoclonal antibody (9G2)



ALX-804-058-C100	100 µg	422.00 USD
Do you need bulk/larger quantities?

Replaces Prod. #: BML-BA1902

Product Details

Clone:	9G2

Host:	Mouse

Isotype:	IgG1

Immunogen:	Recombinant human β-catenin.

UniProt ID:	P35222

Species reactivity:	Human, Mouse Dog

Specificity:	Recognizes exon 3 (α-catenin-binding site) of human, mouse, rat (weak) and dog β-catenin.

Applications:	ELISA, ICC, IP, WB

Recommended Dilutions/Conditions:	Immunocytochemistry (0.1-1µg/ml) Western Blot (0.1µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone (PVP) 0.1% (v/v) Tween 20, 150mM sodium chloride) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Positive Control:	Included. (Prod. No. ALX-840-027)

Purity Detail:	Thiophilic adsorption and size exclusion chromatography purified.

Formulation:	Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.

Reconstitution:	Reconstitute with 1ml water (15 minutes at room temperature).

Use/Stability:	Stable at -80°C up to 1 year, at 4°C up to 3 months.

Handling:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Regulatory Status:	RUO - Research Use Only

β-Catenin monoclonal antibody (8E4) Western blot

Figure A: Western blot analysis of recombinant β-catenin from E. coli (mainly depospho-β-catenin) using various antibodies.
Figure B: Western blot analysis of lysate of pervanadate-treated SKOV-3 cells (phospho- and dephospho-β-catenin) using various antibodies.
Method: β-catenin probes were applied to SDS-PAGE, transferred to PVDF membranes, probed for 1 hour at 15-22°C with the indicated MAbs (0.5µg/ml) and developed with ECL (exposure time 5 sec.). Antibodies used are:
Lane 1: MAb to β-Catenin (dephospho, aa 35-50) (7A7) (Prod. No. ALX-804-259)
Lane 2: MAb to β-Catenin (NT/Exon 2) (7D11) (Prod. No. ALX-804-060)
Lane 3: MAb to β-Catenin (dephospho, aa 27-37) (8E4) (Prod. No. ALX-804-260)
Lane 4: MAb to β-Catenin (Exon 3) (9G2) (Prod. No. ALX-804-058)

Please mouse over

Related Products

	β-Catenin (NT/exon 2) monoclonal antibody (7D11)
	ELISA, IHC, IP, WB \| Print as PDF

	ALX-804-060-C100	100 µg	553.00 USD
Do you need bulk/larger quantities?

	β-Catenin monoclonal antibody (dephospho, aa 35-50) (7A7)
	ELISA, ICC, IHC (PS), IP, WB \| Print as PDF

	ALX-804-259-C100	100 µg	553.00 USD
Do you need bulk/larger quantities?