Replaces Prod. #: BML-SA257
This antibody has been successfully used in Western blot procedures to detect µ-calpain from human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, and rat myoblasts, kidney, liver and spleen. Immunofluorescence staining of µ-calpain in porcine LLC-PK1 cells with ALX-804-050 results in diffuse cytoplasmic staining.
Product Details
| Alternative Name: | Calpain I |
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| Clone: | 9A4H8D3 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Purified bovine µ-calpain. |
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| UniProt ID: | Q27970 |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Hamster, Porcine, Rabbit
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| Specificity: | Recognizes an epitope in domain III (aa 465-520) of µ-calpain from several mammalian sources (e.g. human, mouse, rat, pig, bovine, hamster, rabbit). |
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| Crossreactivity: | Does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin. |
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| Applications: | ICC, IHC, WB
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| Recommended Dilutions/Conditions: | Immunocytochemistry (1:100)
Immunohistochemistry (frozen sections, 1:100)
Western Blot (1:2,000)
Note: Not recommended for immunoprecipitation. |
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| Application Notes: | Detects a band of ~80kDa by Western blot. |
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| Formulation: | Liquid. Ascites containing 0.05% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Reverse phase protein arrays for the identification/validation of biomarkers of beef texture and their use for early classification of carcasses: M. Gagaoua, et al.; Food Chem.
250, 245 (2018),
Application(s): Reverse phase protein array,
Abstract;
Calpain is a major cell death effector in selective striatal degeneration induced in vivo by 3-nitropropionate: implications for Huntington’s disease: N. Bizat, et al.; J. Neurosci.
23, 5020 (2003),
Abstract;
Full Text
Calpain-dependent proteolytic cleavage of the p35 cyclin-dependent kinase 5 activator to p25: G. Kusakawa, et al.; J. Biol. Chem.
275, 17166 (2000),
Abstract;
Full Text
Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains: J. Cong, et al.; J. Biol. Chem.
268, 25740 (1993),
Abstract;
Full Text
A comparison of the intracellular distribution of mu-calpain, m-calpain, and calpastatin in proliferating human A431 cells: R.D. Lane, et al.; Exp. Cell Res.
203, 5 (1992),
Abstract;
