Replaces Prod. #: BML-SA185
Product Details
| Alternative Name: | Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 |
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| Clone: | IID8 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Purified dog cardiac sarcoplasmic reticulum. |
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| UniProt ID: | O46674 |
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| Species reactivity: | Human, Rat
Bovine, Dog, Porcine, Rabbit
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| Specificity: | Recognizes the SERCA2a and SERCA2b isoforms. |
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| Crossreactivity: | Does not cross-react with frog SERCA2 ATPase. |
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| Applications: | ICC, IHC (FS), WB
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| Recommended Dilutions/Conditions: | Immunohistochemistry (frozen sections, 1:500)
Western Blot (1:2,500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~110kDa by Western blot.
For detection of rat SERCA2 ATPase use MAb to SERCA2 ATPase (2A7-A1) (Prod. No. ALX-804-088). |
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| Purity Detail: | Affinity purified. |
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| Formulation: | Liquid. Ascites diluted in PBS containing 0.05% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Localization of endoplasmic reticulum and plasma membrane Ca2+-ATPases in subcellular fractions and sections of pig cerebellum: M.R. Sepulveda, et al.; Eur. J. Neurosci.
19, 542 (2004),
Abstract;
Dual gene therapy with SERCA1 and Kir2.1 abbreviates excitation without suppressing contractility: I.L. Ennis, et al.; J. Clin. Invest.
109, 393 (2002),
Abstract;
Full Text
Low temperature molecular adaptation of the skeletal muscle sarco(endo)plasmic reticulum Ca2+-ATPase 1 (SERCA 1) in the wood frog (Rana sylvatica): L. Dode, et al.; J. Biol. Chem.
276, 3911 (2001),
Abstract;
Biogenesis of endoplasmic reticulum proteins involved in Ca2+ signalling during megakaryocytic differentiation: an in vitro study: C. Lacabaratz-Porret, et al.; Biochem. J.
350, 723 (2000),
Abstract;
Full Text
Control of sarcoplasmic/endoplasmic-reticulum Ca2+ pump expression in cardiac and smooth muscle: C.M. Misquitta, et al.; Biochem. J.
338, 167 (1999),
Abstract;
Full Text
Functional characterization of alternatively spliced human SERCA3 transcripts: E. Poch, et al.; Am. J. Physiol.
275, C1449 (1998),
Abstract;
Full Text
Transcriptional regulation of phospholamban gene and translational regulation of SERCA2 gene produces coordinate expression of these two sarcoplasmic reticulum proteins during skeletal muscle phenotype switching: P. Hu, et al.; J. Biol. Chem.
270, 11619 (1995),
Abstract;
Full Text
Correlated expression of the 97 kDa sarcoendoplasmic reticulum Ca(2+)-ATPase and Rap1B in platelets and various cell lines: C. Magnier, et al.; Biochem. J.
297, 343 (1994),
Abstract;
Differential immunohistochemical localization of inositol 1,4,5-trisphosphate- and ryanodine-sensitive Ca2+ release channels in rat brain: A.H. Sharp, et al.; J. Neurosci.
13, 3051 (1993),
Abstract;
In situ hybridization and immunocytochemical localization of SERCA2 encoded Ca2+ pump in rabbit heart and stomach: K.M. Mearow, et al.; Mol. Cell. Biochem.
121, 155 (1993),
Abstract;
Immunological relatedness of the sarcoplasmic reticulum Ca(2+)-ATPase and the Na+,K(+)-ATPase: E. Molnar, et al.; Biochim. Biophys. Acta
1103, 281 (1992),
Abstract;
A study of the organellar Ca2(+)-transport ATPase isozymes in pig cerebellar Purkinje neurons: L. Plessers, et al.; J. Neurosci.
11, 650 (1991),
Abstract;
Full Text
Ca-ATPase isozyme expression in sarcoplasmic reticulum is altered by chronic stimulation of skeletal muscle: F.N. Briggs, et al.; FEBS Lett.
259, 269 (1990),
Abstract;
Identification and characterization of proteins in sarcoplasmic reticulum from normal and failing human left ventricles: M.A. Movsesian, et al.; J. Mol. Cell. Cardiol.
22, 1477 (1990),
Abstract;
A monoclonal antibody to the Ca2+ ATPase of cardiac sarcoplasmic reticulum cross-reacts with slow type I but not with fast type II canine skeletal muscle fibers: an immunocytochemical study: A.O. Jorgensen, et al.; Cell Motil. Cytoskeleton
9, 164 (1988),
Abstract;
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