| ALX-804-047-R050 | 50 µl | 513.00 USD |  |
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| Alternative Name: | Heat shock protein 70 |
| Clone: | 3A3 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Recombinant human HSP70 (heat shock protein 70). |
| UniProt ID: | P0DMV8 (HSPA1A), P0DMV9 (HSPA1B) |
| GenBank ID: | M11717 |
| Gene/Protein Identifier: | NP_005336.3 (RefSeq), NM_005345 (RefSeq), 3303 (Entrez GeneID), 140550 (OMIM) |
| Species reactivity: | Human, Mouse, Rat Amphibian, Arthropod, Avian, Drosophila, Fish, Porcine, Plant, Primate, Yeast, Mollusc |
| Specificity: | Recognizes several members of the HSP70 gene family including HSP70, HSC70, p75, and following heat shock, HSP72. |
| Crossreactivity: | HSP70, HSC70, HSP75, HSP72 (following heat shock). |
| Applications: | ICC, IF, IHC (PS), IP, WB Gel Shift |
| Recommended Dilutions/Conditions: | Immunocytochemistry (1:100) Immunofluorescence (1:50-1:200) Immunohistochemistry (paraffin sections) (1:200) Immunoprecipitation (2µl) Western Blot (1:1,000-1:5,000) Gel Shift (Assay dependent) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Immunocytochemistry: staining of HSP70 in heat shocked HeLa cells results in cytoplasmic staining Detects bands from ~70kDa to ~78kDa by Western blot representing different members of the HSP70 family. |
| Formulation: | Liquid. Ascites diluted in PBS containing 0.05% sodium azide. |
| Handling: | Avoid freeze/thaw cycles. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. |
| Technical Info/Product Notes: | 2-dimensional gel electrophoresis is required to resolve the heat induced form of these proteins from their constitutively expressed counterparts. |
| Regulatory Status: | RUO - Research Use Only |
