Replaces Prod. #: BML-SA245
Product Details
Alternative Name: | RyR |
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Clone: | 34C |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Partially purified chicken pectoral muscle RyR (ryanodine receptor). |
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UniProt ID: | Q90984 |
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Species reactivity: | Human, Mouse, Rat Amphibian, Bovine, Dog, Fish, Mink, Monkey, Rabbit, Sheep
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Specificity: | Recognizes RyR-1 and RyR-2 isoforms. In chicken, it detects the α, β and cardiac isoforms; in frog the α and β isoforms; and in fish the α isoform. Also recognizes RyR-3 in mouse cells. |
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Applications: | ICC, IHC (FS), IP, WB
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Recommended Dilutions/Conditions: | Immunohistochemistry (frozen sections, 1:1,000) Western Blot (1:5,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~565kDa by Western blot, representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565kDa, representing the α and β isoforms. |
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Formulation: | Liquid. Contains 0.05% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
A transgenic myogenic cell line lacking ryanodine receptor protein for homologous expression studies: reconstitution of Ry1R protein and function: R.A. Moore, et al.; J. Cell. Biol.
140, 843 (1998),
Abstract;
Role of ryanodine receptors in the assembly of calcium release units in skeletal muscle: F. Protasi, et al.; J. Cell. Biol.
140, 831 (1998),
Abstract;
Nonmammalian vertebrate skeletal muscles express two triad junctional foot protein isoforms: E.B. Olivares, et al.; Biophys. J.
59, 1153 (1991),
Abstract;
Ryanodine and inositol triphosphate receptors coexist in avian cerebellar Purkinje neurons: P.D. Walton, et al.; J. Cell. Biol.
113, 1145 (1991),
Abstract;
Identification and localization of two triad junctional foot protein isoforms in mature avian fast twitch skeletal muscle: J.A. Airey, et al.; J. Biol. Chem.
265, 14187 (1990),
Abstract;
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