PDI monoclonal antibody (RL90)



ALX-804-012-R100	100 µl	753.00 USD
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Product Details

Alternative Name:	Protein disulfide-isomerase

Clone:	RL90

Host:	Mouse

Isotype:	IgG2a

Immunogen:	Purified rat PDI (protein disulfide-isomerase).

UniProt ID:	P04785

Species reactivity:	Human, Mouse, Rat Hamster, Porcine

Applications:	Flow Cytometry, ICC, IHC (FS), IHC (PS), IP, WB, FUNC

Recommended Dilutions/Conditions:	Immunocytochemistry (1:100) Immunohistochemistry (frozen sections, 1:100) Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~59kDa (rat liver extract) or ~61kDa (human liver extract) by Western blot.

Purity Detail:	Protein A-affinity purified.

Formulation:	Liquid. Purified antibody containing 1mg/ml BSA and 0.05% sodium azide.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Shipped on Blue Ice

Long Term Storage:	-20°C

Scientific Background:	The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide-Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

Technical Info/Product Notes:	Immunohistochemical staining of PDI in rat intestine with Prod. No. ALX-804-012 yields a pattern consistent with cytoplasmic staining. In immunoprecipitation procedures, Prod. No. ALX-804-012 has been shown to inhibit the activity of PDI in vitro. Prod. No. ALX-804-012 has also been found to inhibit disulfide bond reduction of the HIV protein, gp120, at the cell surface of CHO cells and human lymphoid cells.

Regulatory Status:	RUO - Research Use Only

Product Literature References

Collagen Accumulation in Osteosarcoma Cells lacking GLT25D1 Collagen Galactosyltransferase: S. Baumann & T. Hennet; J. Biol. Chem. 291, 18514 (2016), Application(s): Immunofluorescence, Abstract; Full Text

Coronavirus envelope (E) protein remains at the site of assembly: P. Venkatagopalan, et al.; Virology 478C, 75 (2015), Application(s): Immunofluorescence, Abstract;

Sustained integrin ligation involves extracellular free sulfhydryls and enzymatically catalyzed disulfide exchange: J. Lahav, et al.; Blood 100, 2472 (2002), Abstract; Full Text

Inhibition of human immunodeficiency virus infection by agents that interfere with thiol-disulfide interchange upon virus-receptor interaction: H.J. Ryser, et al.; PNAS 91, 4559 (1994), Abstract; Full Text

Inhibition of a reductive function of the plasma membrane by bacitracin and antibodies against protein disulfide-isomerase: R. Mandel, et al.; PNAS 90, 4112 (1993), Abstract; Full Text

Protein disulphide-isomerase from human placenta and rat liver. Purification and immunological characterization with monoclonal antibodies: C.S. Kaetzel, et al.; Biochem. J. 241, 39 (1987), Abstract;