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FasL (mouse) monoclonal antibody (H11)

ALX-804-010-C100 100 µg Purified 393.00 USD
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Product Details

Alternative Name:CD178, CD95L, APO-1L, TNFSF 6
Immunogen:Synthetic peptide corresponding to aa 196-220 of mouse FasL (CD95L; APO-1L; CD178).
UniProt ID:P41047
Source:Purified from hybridoma tissue culture supernatant.
Species reactivity:Mouse
Applications:Flow Cytometry, ICC, WB
Application Notes:For immunohistochemistry, we recommend MAb to FasL (A11) (Prod. No. ALX-804-009).
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Use/Stability:Stable for at least 6 months after receipt when stored at +4°C undiluted.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Regulatory Status:RUO - Research Use Only
FasL (mouse) monoclonal antibody (H11) (biotin conjugate) Schematic structure of immunogen
Schematic structure of mouse FasL (peptide, aa 196-220)
FasL (mouse) monoclonal antibody (H11) WB
Figure: Detection of FasL in 293T cells transfected with a FasL expression plasmid (right panel). Mock-transfected cells (left panel).
Method: Cells extracts from (2 x 106) cells transfected with a FasL-expression plasmid were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-FasL H11 antibody at 2µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG (ALX-211-052) and a chemiluminescence detection system. Arrow indicates FasL."
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FasL (mouse) monoclonal antibody (H11) (biotin conjugate) Schematic structure of immunogen FasL (mouse) monoclonal antibody (H11) WB

Product Literature References

Immature and neurally differentiated mouse embryonic stem cells do not express a functional Fas/Fas ligand system: G. Brunlid, et al.; Stem Cells 25, 2551 (2007), Abstract; Full Text
Transgenic expression of CD95 ligand on thyroid follicular cells confers immune privilege upon thyroid allografts: L. Tourneur, et al.; J. Immunol. 167, 1338 (2001), Abstract;
CD4+ T cells reactivated with superantigen are both more sensitive to FasL-mediated killing and express a higher level of FasL: J.K. Wang, et al.; Cell. Immunol. 179, 153 (1997), Abstract;
Characterization of the non-functional Fas ligand of gld mice: M. Hahne, et al.; Int. Immunol. 7, 1381 (1995), Abstract;

General Literature References

Apoptosis by death factor: S. Nagata; Cell 88, 355 (1997), Abstract;
A role for CD95 ligand in preventing graft rejection: D. Bellgrau, et al.; Nature 377, 630 (1995), Abstract;
Fas ligand-induced apoptosis as a mechanism of immune privilege: T.S. Griffith, et al.; Science 270, 1189 (1995), Abstract;
Cytolytic T-cell cytotoxicity is mediated through perforin and Fas lytic pathways: B. Lowin, et al.; Nature 370, 650 (1994), Abstract;
Mature T cells of autoimmune lpr/lpr mice have a defect in antigen-stimulated suicide: H. Russell, et al.; PNAS 90, 4409 (1993), Abstract; Full Text

Related Products

FasL (mouse) monoclonal antibody (A11) 

Purified from hybridoma tissue culture supernatant., Flow Cytometry, IHC (FS), IHC (PS) | Print as PDF
ALX-804-009-C100 100 µg 372.00 USD
Do you need bulk/larger quantities?

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