Product Details
| Alternative Name: | CD178, CD95L, APO-1L, TNFSF 6 |
| |
| Clone: | H11 |
| |
| Host: | Rat |
| |
| Isotype: | IgG2a |
| |
| Immunogen: | Synthetic peptide corresponding to aa 196-220 of mouse FasL (CD95L; APO-1L; CD178). |
| |
| UniProt ID: | P41047 |
| |
| Source: | Purified from hybridoma tissue culture supernatant. |
| |
| Species reactivity: | Mouse
|
| |
| Applications: | Flow Cytometry, ICC, WB
|
| |
| Application Notes: | For immunohistochemistry, we recommend MAb to FasL (A11) (Prod. No. ALX-804-009). |
| |
| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
| |
| Use/Stability: | Stable for at least 6 months after receipt when stored at +4°C undiluted. |
| |
| Handling: | Avoid freeze/thaw cycles. |
| |
| Shipping: | Blue Ice Not Frozen |
| |
| Long Term Storage: | +4°C |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Schematic structure of mouse FasL (peptide, aa 196-220)
Figure: Detection of FasL in 293T cells transfected with a FasL expression plasmid (right panel). Mock-transfected cells (left panel).
Method: Cells extracts from (2 x 10
6) cells transfected with a FasL-expression plasmid were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-FasL H11 antibody at 2µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG (
ALX-211-052) and a chemiluminescence detection system. Arrow indicates FasL."
Please mouse over
Product Literature References
Immature and neurally differentiated mouse embryonic stem cells do not express a functional Fas/Fas ligand system: G. Brunlid, et al.; Stem Cells
25, 2551 (2007),
Abstract;
Full Text
Transgenic expression of CD95 ligand on thyroid follicular cells confers immune privilege upon thyroid allografts: L. Tourneur, et al.; J. Immunol.
167, 1338 (2001),
Abstract;
CD4+ T cells reactivated with superantigen are both more sensitive to FasL-mediated killing and express a higher level of FasL: J.K. Wang, et al.; Cell. Immunol.
179, 153 (1997),
Abstract;
Characterization of the non-functional Fas ligand of gld mice: M. Hahne, et al.; Int. Immunol.
7, 1381 (1995),
Abstract;
General Literature References
Apoptosis by death factor: S. Nagata; Cell
88, 355 (1997),
Abstract;
A role for CD95 ligand in preventing graft rejection: D. Bellgrau, et al.; Nature
377, 630 (1995),
Abstract;
Fas ligand-induced apoptosis as a mechanism of immune privilege: T.S. Griffith, et al.; Science
270, 1189 (1995),
Abstract;
Cytolytic T-cell cytotoxicity is mediated through perforin and Fas lytic pathways: B. Lowin, et al.; Nature
370, 650 (1994),
Abstract;
Mature T cells of autoimmune lpr/lpr mice have a defect in antigen-stimulated suicide: H. Russell, et al.; PNAS
90, 4409 (1993),
Abstract;
Full Text
Related Products
