Product Details
Alternative Name: | Tubulin polymerization-promoting protein, p25 |
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Clone: | 17C6 |
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Host: | Rat |
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Isotype: | IgG2a |
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Immunogen: | Full lenght recombinant TPPP. |
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UniProt ID: | O94811 |
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Source: | Purified from concentrated hybridoma tissue culture supernatant. |
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Species reactivity: | Human, Mouse Monkey
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Specificity: | Recognizes full-length endogenous TPPP/p25. |
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Applications: | ICC, IHC (PS), WB
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Recommended Dilutions/Conditions: | Immunocytochemistry (10µg/ml) Immunohistochemistry (paraffin sections, 10µg/ml) Western Blot (200ng/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Protein G affinity purified. |
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Formulation: | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
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Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
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Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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Shipping: | Shipped on Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Scientific Background: | TPPP plays a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. Plays a role in mitotic spindle assembly and nuclear envelope breakdown. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
The phosphorylation of p25/TPPP by LIM kinase 1 inhibits its ability to assemble microtubules: K. Acevedo, et al.; Exp. Cell Res.
313, 4091 (2007),
Abstract;
General Literature References
Tubulin polymerization promoting protein (TPPP/p25) as a marker for oligodendroglial changes in multiple sclerosis: R. Hofberger, et al.; Glia
58, 1847 (2010),
Abstract;