Product Specification
Alternative Name: | β-1,4-Galactosyltransferase 1, GGTB2 |
|
Clone: | GT2/36/118 |
|
Host: | Mouse |
|
Isotype: | IgG2b |
|
Immunogen: | Recombinant human B4GALT1 |
|
UniProt ID: | P15291 |
|
Species reactivity: | Human
|
|
Crossreactivity: | Does not cross-react with mouse B4GALT1. |
|
Applications: | ICC, WB
|
|
Recommended Dilutions/Conditions: | Immunocytochemistry (1:500) Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
|
Purity Detail: | Protein G-affinity purified. |
|
Formulation: | Liquid. Contains 150µg/ml BSA. |
|
Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
|
Shipping: | Shipped on Blue Ice |
|
Short Term Storage: | +4°C |
|
Long Term Storage: | -20°C |
|
Scientific Background: | β-1,4-Galactosyltransferase 1 (B4GALT1) is one of seven B4GALT genes. Human B4GALT1 encodes a type II membrane protein, which is ubiquitously expressed and involved in glycoconjugate biosynthesis. A soluble form (aa 78 to 398), derived from proteolytic cleavage, is a component of lactose synthase in milk that also contains α-lactalbumin. The amino acid sequence of human B4GALT1 is 88%, 87% and 71% identical to that of rat, mouse/canine and chicken.Defects in B4GALT1 are the cause of multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. |
|
Regulatory Status: | RUO - Research Use Only |
|
Figure: Immunocytochemistry using B4GALT1, mAb (GT2/36/118) (Prod. No. ALX-803-339). The specificity of the monoclonal antibody was confirmed by immunofluorescence staining of the Golgi apparatus in HeLa cells. Method: B4GALT1, mAb (GT2/36/118) was diluted 1:500 and applied to saponin-permeabilized HeLa cells (after fixation in 3% paraformadehyde). A goat-anti-mouse Alexa488 antibody was used as secondary antibody. DAPI was used to stain the nuclei. The Golgi apparatus shows as a typical perinuclear structure.
Please mouse over
Product Literature References
Golgi function and dysfunction in the first COG4-deficient CDG type II patient: E. Reynders, et al.; Hum. Mol. Gen.
18, 3244 (2009),
Abstract;
Clathrin-dependent Association of CVAK104 with Endosomes and the Trans-Golgi Network: M. Düwel & E.J. Ungewickell; Mol. Biol. Cell
17, 4513 (2006),
Abstract;
Golgi Inheritance in Mammalian Cells Is Mediated through Endoplasmic Reticulum Export Activities: N. Altan-Bonnet; Mol. Biol. Cell
17, 990 (2006),
Abstract;
Tumor Necrosis Factor-α Up-regulates the Expression of β1,4-Galactosyltransferase I in Primary Human Endothelial Cells by mRNA Stabilization: J.J. Garcia-Vallejo, et al.; J. Biol. Chem.
280, 12676 (2005),
Abstract;
The Palmitoyltransferase of the Cation-dependent Mannose 6-Phosphate Receptor Cycles between the Plasma Membrane and Endosomes: J. Stöckli & J. Rohrer; Mol. Biol. Cell
15, 2617 (2004),
Abstract;
SseG, a virulence protein that targets Salmonella to the Golgi network: S.P. Salcedo & D.W. Holden; EMBO J.
22, 5003 (2003),
Abstract;
Lysosomal Hydrolase Mannose 6-Phosphate Uncovering Enzyme Resides in the trans-Golgi Network: J. Rohrer & R. Kornfeld; Mol. Biol. Cell
12, 1623 (2001),
Abstract;
Dynamics of Transitional Endoplasmic Reticulum Sites in Vertebrate Cells: A.T. Hammond & B.S. Glick; Mol. Biol. Cell
11, 3013 (2000),
Abstract;
Endoplasmic reticulum-to-cytosol transport of free polymannose oligosaccharides in permeabilized HepG2 cells: S.E.H. Moore, et al.; EMBO J.
14, 6034 (1995),
Abstract;
Kin recognition between medial Golgi enzymes in HeLa cells: T. Nilsson, et al.; EMBO J.
13, 562 (1994),
Abstract;
The TGN38 Glycoprotein Contains Two Non-Overlapping Signals That Mediate Localization to the Trans-Golgi Network: S. Ponnambalam, et al.; J. Cell Biol.
125, 253 (1994),
Abstract;
Giantin, a Novel Conserved Golgi Membrane Protein Containing a Cytoplasmic Domain of at Least 350 kDa: A.D. Linstedt & HP. Hauri; Mol. Biol. Cell
4, 679 (1993),
Abstract;
Monoclonal antibodies to soluble human milk galactosyltransferase (lactose synthase A protein): E.G. Berger, et al.; Carbohydr. Res.
149, 23 (1986),
Abstract;