Alternative Name: | Chondroitin sulfate proteoglycan |
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Clone: | 5C5 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Human articular cartilage aggrecan (chondroitinase ABC/keratanase II/endo-β-galactosidase digested). |
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UniProt ID: | P16112 |
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Species reactivity: | Human Bovine
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Specificity: | Recognizes an epitope within the globular region of aggrecan. |
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Crossreactivity: | Does not cross-react with rat, chicken or shark aggrecan. |
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Applications: | ELISA, IHC (PS), IP, WB
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Recommended Dilutions/Conditions: | ELISA (1:500-1:1,000) Immunohistochemistry (paraffin sections, 1:50-1:300; predigestion of tissue sections with 100-150mU/ml testicular hyaluronidase or rather crude collagenases (1-2 hours at 37°C) may improve staining) Immunoprecipitation (1:50-1:300) Western Blot (1:100-1:300; reducing and non-reducing conditions) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Formulation: | Liquid. Ascites. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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