Clone: | 378D5 |
|
Host: | Mouse |
|
Isotype: | IgG1 |
|
Immunogen: | Collagen type XII purified from bovine kidney. |
|
Species reactivity: | Human, Mouse, Rat Bovine, Chicken
|
|
Applications: | ELISA, IHC (FS), IP, WB
|
|
Recommended Dilutions/Conditions: | ELISA (1:500-1:1,000) Immunohistochemistry (frozen sections, 1:500-1:1,000; a mild trypsin- or Protease 27 (Sigma)-digestion (0.05% for 10-15 min at 37°C) or alternatively microwave oven treatment is recommended to unmask the collagens) Immunoprecipitation (1:50-1:100) Western Blot (1:100-1:300) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
|
Application Notes: | Not recommended for IHC (PS). |
|
Formulation: | Liquid. Ascites. Contains no preservatives. |
|
Handling: | Avoid freeze/thaw cycles. |
|
Shipping: | Shipped on Blue Ice |
|
Short Term Storage: | +4°C |
|
Long Term Storage: | -20°C |
|
Regulatory Status: | RUO - Research Use Only |
|