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LC3 monoclonal antibody (5H3)

ALX-803-082-C100 100 µg 699.00 USD
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Product Details

Alternative Name:Microtubule-associated protein 1 light chain 3, MAP1LC3
Immunogen:Synthetic peptide corresponding to an internal sequence identical in LC3A, LC3B and LC3C conjugated to hemocyanin.
UniProt ID:Q9H492 (LC3A), Q9GZQ8 (LC3B), Q9BXW4 (LC3C)
Species reactivity:Human
Specificity:Recognizes both forms of endogenous LC3.
Applications:ICC, IHC, WB
Recommended Dilutions/Conditions:Immunocytochemistry (paraformaldehyde/methanol fixation; 1-10 µg/ml)
Western Blot (0.5µg/ml for HRPO/ECL detection; Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)
We strongly recommend to use PVDF membranes for immunoblot assays.
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~18kDa (LC3-I; cytoplasmic form) and a band of ~16kDa (LC3-II; lipidated form) by Western blot.
Positive Control:Included. (Prod. No. ALX-840-038)
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
Formulation:Liquid. In PBS containing 50% glycerol, 0.09% sodium azide, PEG and sucrose.
Use/Stability:Stable at -20°C up to 1 year.
Handling:Thawed aliquots may be stored at 4°C up to 3 months. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.
Regulatory Status:RUO - Research Use Only

Product Literature References

DRAM1 Promotes Lysosomal Delivery of Mycobacterium marinum in Macrophages: A.B. Karp, et al.; Cells 12, 828 (2023), Abstract;
SERTAD1 Sensitizes Breast Cancer Cells to Doxorubicin and Promotes Lysosomal Protein Biosynthesis: H.A. Nguyen, et al.; Biomedicines 10, 1148 (2022), Abstract;
Conidiobolus coronatus induces oxidative stress and autophagy response in Galleria mellonella larvae: M. Kazek, et al.; PLoS One 15, e0228407 (2020), Abstract; Full Text

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