Product Details
| Alternative Name: | GAD65, GAD2 |
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| Clone: | 83/2E6 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant human GAD65 (glutamate decarboxylase (65kDa isoform)). |
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| UniProt ID: | Q05329 |
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| Species reactivity: | Human, Rat
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| Specificity: | Recognizes an epitope (aa 221-442) of human GAD65. Binds to human and rat pancreas tissue but not mouse pancreas tissue. Not detectable on Bouin-fixed pancreatic tissues. No binding to viable rat islet cells. |
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| Crossreactivity: | Does not cross-react with GAD67. |
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| Applications: | ICC, IHC (FS), IP
Radioimmunoassay
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| Application Notes: | GAD-binding of this antibody is competitively inhibited by human GAD-antibodies. |
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| Purity Detail: | Ammonium sulphate precipitation. |
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| Formulation: | Liquid. In PBS. pH 7.4, containing 0.9mg/ml sodium azide. |
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| Use/Stability: | Stable for at least 1 year when stored at +4°C. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
A monoclonal antibody-based characterization of autoantibodies against glutamic acid decarboxylase in adults with latent autoimmune diabetes: B. Ziegler, et al.; Autoimmunity
28, 61 (1998),
Abstract;
Immunohistochemical differentiation of monoclonal GAD antibodies recognizing linear or conformational epitope regions: P. Augstein, et al.; Pancreas
15, 139 (1997),
Abstract;
Comparison of the islet cell antibody pattern of monoclonal glutamic acid decarboxylase antibodies recognizing linear and conformational epitopes: P. Augstein, et al.; Acta Histochem.
98, 229 (1996),
Abstract;
Monoclonal antibodies specific to the glutamic acid decarboxylase 65 kDa isoform derived from a non-obese diabetic (NOD) mouse: B. Ziegler, et al.; Diabetes Res.
25, 47 (1994),
Abstract;
