Product Details
| Clone: | K14D10 |
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| Host: | Mouse |
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| Isotype: | IgG2a |
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| Immunogen: | Pig proinsulin. |
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| Species reactivity: | Rat
|
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| Specificity: | Recognizes surface structure of viable isolated rat beta cells. Does not recognize mouse islet beta cells. |
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| Crossreactivity: | Cross-reactivity with human pancreatic beta cells was not detected. |
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| Applications: | Flow Cytometry, ICC, IHC (FS)
Cell sorting
|
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| Application Notes: | Not recommended for WB and IP. |
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| Purity Detail: | Ammonium sulphate precipitation. |
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| Formulation: | Liquid. In PBS, pH 7.4, containing 0.9mg/ml sodium azide. |
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| Use/Stability: | Stable for at least 1 year when stored at +4°C. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Immunomagnetic purification of beta cells from rat islets of Langerhans: V. Hadjivassiliou, et al.; Diabetologia
43, 1170 (2000),
Abstract;
Recognition of islet-directed peripheral blood lymphocytes in vitro before rejection of allogeneic grafted islets: B. Kuttler, et al.; Transplantation
69, 1987 (2000),
Abstract;
Co-culture of pancreatic islets and allogeneic lymphocytes: alterations of responder and stimulator cells: B. Kuttler, et al.; Transplantation
64, 480 (1997),
Abstract;
Monoclonal antibody-mediated cytotoxicity against rat beta cells detected in vitro does not cause beta-cell destruction in vivo: B. Ziegler, et al.; Diabetologia
35, 608 (1992),
Abstract;
Influence of target cell preparation on binding of monoclonal islet cell reactive antibodies (mc-ICRA) in cellular enzyme-linked immunosorbent assay (CELISA): M. Schlosser, et al.; J. Immunol. Methods
140, 101 (1991),
Abstract;
