Product Details
| Clone: | MON-152 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant human furin. |
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| UniProt ID: | P09958 |
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| Source: | Ascites fluid from Balb/c mice as obtained after injection of MON-152 producing hybridoma cells and isolated using a physiological salt solution (0.9% NaCl). |
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| Species reactivity: | Human
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| Specificity: | Recognizes an epitope in the cysteine-rich region of human furin convertase. |
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| Crossreactivity: | Does not cross-react with mouse furin convertase. |
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| Applications: | ICC, IP, WB
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| Recommended Dilutions/Conditions: | Immunocytochemistry (1:200-1:400)
Immunoprecipitation (1:200; 50mM TRIS/HCl, pH 7.4, 150mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Formulation: | Liquid. Ascites. Contains no preservatives. |
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| Use/Stability: | Stable for at least 3 months when stored at +4°C. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Mechanism and significance of cell type-dependent neutralization of flaviviruses: S. Mukherjee, et al.; J. Virol.
88, 7210 (2014),
Application(s): Western blot,
Abstract;
Full Text
Proprotein convertase FURIN constrains Th2 differentiation and is critical for host resistance against Toxoplasma gondii: A. Oksanen, et al.; J. Immunol.
193, 5470 (2014),
Abstract;
Proprotein convertase furin enhances survival and migration of vascular smooth muscle cells via processing of pro-nerve growth factor: D. Urban, et al.; J. Biochem.
153, 197 (2013),
Abstract;
Proprotein convertase subtilisin/kexin type 3 promotes adipose tissue-driven macrophage chemotaxis and is increased in obesity: K. Kappert, et al.; PLoS One
8, e70542 (2013),
Application(s): Western blot,
Abstract;
Full Text
Generation and characterization of non-competitive furin-inhibiting nanobodies: J. Zhu, et al.; Biochem. J.
448, 73 (2012),
Application(s): IP,
Abstract;
Full Text
Proprotein convertases in human atherosclerotic plaques: the overexpression of FURIN and its substrate cytokines BAFF and APRIL: H. Turpeinen, et al.; Atherosclerosis
219, 799 (2011),
Application(s): Immunohistochemistry,
Abstract;
Full-length, membrane-anchored TWEAK can function as a juxtacrine signaling molecule and activate the NF-kappaB pathway: S.A. Brown, et al.; J. Biol. Chem.
285, 17432 (2010),
Application(s): Western blot,
Abstract;
Full Text
IGF-1 increases macrophage motility via PKC/p38-dependent alphavbeta3-integrin inside-out signaling: V. Furundzija, et al.; Biochem. Biophys. Res. Commun.
394, 786 (2010),
Application(s): Western blot,
Abstract;
Post-endoplasmic reticulum rescue of unstable MHC class I requires proprotein convertase PC7: R.M. Leonhardt, et al.; J. Immunol.
184, 2985 (2010),
Application(s): Immunofluorescence,
Abstract;
Full Text
Cell-surface processing of pro-ADAMTS9 by furin: B.H. Koo, et al.; J. Biol. Chem.
281, 12485 (2006),
Abstract;
Full Text
The contribution of arginine residues within the P6-P1 region of alpha 1-antitrypsin to its reaction with furin: E.K. Dufour, et al.; J. Biol. Chem.
276, 38971 (2001),
Abstract;
Full Text
Processing, stability, and receptor binding properties of oligomeric envelope glycoprotein from a primary HIV-1 isolate: I. Staropoli, et al.; J. Biol. Chem.
275, 35137 (2000),
Abstract;
Full Text
Biosynthesis, distinct post-translational modifications, and functional characterization of lymphoma proprotein convertase: J.W. van de Loo; J. Biol. Chem.
272, 27116 (1997),
Abstract;
Full Text
Endoproteolytic cleavage of its propeptide is a prerequisite for efficient transport of furin out of the endoplasmic reticulum: J.W. Creemers, et al.; J. Biol. Chem.
270, 2695 (1995),
Abstract;
Full Text
Modulation of furin-mediated proprotein processing activity by site- directed mutagenesis: J.W. Creemers, et al.; J. Biol. Chem.
268, 21826 (1993),
Abstract;
Full Text
Structure and function of eukaryotic proprotein processing enzymes of the subtilisin family of serine proteases: W.J. Van de Ven, et al.; Crit. Rev. Oncog.
4, 115 (1993),
Abstract;
Development and characterization of a panel of monoclonal antibodies against the novel subtilisin-like proprotein processing enzyme furin: H.L. van Duijnhoven, et al.; Hybridoma
11, 71 (1992),
Abstract;
