Product Details
Alternative Name: | GR |
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Clone: | BuGR2 |
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Host: | Mouse |
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Isotype: | IgG2 |
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Immunogen: | Partially purified rat GR (glucocorticoid receptor). |
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UniProt ID: | P06536 |
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Species reactivity: | Human, Mouse, Rat Guinea pig, Rabbit, Sheep, Yeast
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Specificity: | Recognizes full-lengh GR, the 17kDa DNA binding trypsin fragment and the 45kDa steroid- and DNA-binding chymotrypsin fragment. |
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Crossreactivity: | Does not cross-react with primate, avian or amphibian GR. |
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Applications: | Flow Cytometry, ICC, IHC (PS), IP, WB Gel Shift
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Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections, 5µg/ml) Western Blot (5µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Immunocytochemical staining of GR in L929 cells with Prod. No. ALX-803-006 results in staining of both the cytoplasm and nucleus, even in the presence of hormone.
Detects a band of ~97kDa by Western blot. |
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Formulation: | Lyophilized from PBS, pH 7.2, containing 0.05% sodium azide. |
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Reconstitution: | Reconstitute with distilled water. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Figure: Western blot analysis of glucocorticoid receptor on mouse liver extract using Prod. No.
ALX-803-006."
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Product Literature References
Glucocorticoid receptor/signal transducer and activator of transcription 5 (STAT5) interactions enhance STAT5 activation by prolonging STAT5 DNA binding and tyrosine phosphorylation: S.L. Wyszomierski, et al.; Mol. Endocrinol.
13, 330 (1999),
Abstract;
Full Text
Analysis of FKBP51/FKBP52 chimeras and mutants for Hsp90 binding and association with progesterone receptor complexes: R.L. Barent, et al.; Mol. Endocrinol.
12, 342 (1998),
Abstract;
Full Text
Disruption of the glucocorticoid receptor assembly with heat shock protein 90 by a peptidic antiglucocorticoid: H.P. Dao-Phan, et al.; Mol. Endocrinol.
11, 962 (1997),
Abstract;
Full Text
Phosphorylated sites within the functional domains of the approximately 100-kDa steroid-binding subunit of glucocorticoid receptors: L.I. Smith, et al.; Biochemistry
28, 4490 (1989),
Abstract;
Analysis of the disruptive action of an epididymal protease and the stabilizing influence of molybdate on nondenatured and denatured glucocorticoid receptor: W.J. Hendry, 3rd, et al.; Endocrinology
120, 629 (1987),
Abstract;