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Estrogen receptor α monoclonal antibody (33)

ALX-803-004-C050 50 µg 723.00 USD
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Product Details

Alternative Name:ERα
Immunogen:Synthetic peptide corresponding to aa 247-261 (E247VGMMKGGIRKDRRG261) of the DNA binding domain of ERα (estrogen receptor α). This sequence is completely conserved in many species including mouse, rat, pig, bovine, sheep, chicken and frog.
UniProt ID:P03372
Species reactivity:Human, Mouse, Rat
Bovine, Monkey
Specificity:Recognizes both the steroid occupied and unoccupied forms of ERα.
Crossreactivity:Does not cross-react with androgen receptor, PR (progesterone receptor), GR (glucocorticoid receptor) or ERβ.
Applications:Flow Cytometry, ICC, IF, IHC (FS), IHC (PS), IP, WB
Recommended Dilutions/Conditions:Immunofluorescence (10µg/mL)
Immunohistochemistry (1:20-1:100)
Western Blot (4 µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Immunohistochemistry: Staining of ERα in mouse Leydig cells results in both nuclear and cytoplasmic staining.
Detects a band of ~67kDa by Western blot.
Does not inhibit binding of ER to nuclei and DNA.
Formulation:Liquid. In Dulbecco's PBS, containing 0.05% sodium azide, 1mg/mL BSA and 30% glycerol.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:Steroid receptors are a large superfamily of ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Estrogen receptor (ER) has been classified into two distinct isoforms, α and β. ERα is known to interact in a ligand dependent and independent manner with several known coactivators including Receptor-Interacting Protein 140 (RIP140/ERAP140), ERAP160, and Steroid Receptor Coactivator-1 (SRC-1) to enhance transcriptional activity of target genes.
Regulatory Status:RUO - Research Use Only
Estrogen receptor α monoclonal antibody (33) Western blot
Figure: Western blot of recombinant ERa using MAb to Estrogen Receptor α (33) (Prod. No. ALX-803-004)."
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Estrogen receptor α monoclonal antibody (33) Western blot

Product Literature References

Immunohistochemical localization of oestrogen receptor alpha in the various cell categories of chick embryo ovary: A. Civinini, et al.; Anat. Histol. Embryol. 39, 546 (2010), Application(s): Immunohistochemistry using chick embryo ovary, Abstract;
Histochemical and electrophysiological evidence for estrogen receptors on cultured astrocytes: colocalization with cholinergic receptors: E. Hosli, et al.; Int. J. Dev. Neurosci. 18, 101 (2000), Abstract;
Measurement of estrogen receptors in intact cells by flow cytometry: S. Cao, et al.; Cytometry 41, 109 (2000), Abstract;
Cellular localization of estrogen receptors on neurones in various regions of cultured rat CNS: coexistence with cholinergic and galanin receptors: E. Hosli & L. Hosli; Int. J. Dev. Neurosci. 17, 317 (1999), Abstract;
Development and characterization of monoclonal antibodies to a specific domain of human estrogen receptor: A. Traish, et al.; Steroids 55, 196 (1990), Abstract;

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