Product Details
Alternative Name: | nAChR |
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Clone: | 88B |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Purified Torpedo californica AChR (acetylcholine receptor). |
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Species reactivity: | Mouse, Rat Chicken, Fish, Frog, Torpedo californica
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Specificity: | Recognizes the intracellular domain of skeletal muscle nAChR γ and δ subunits in Torpedo californica and the δ subunit in mouse, rat, chicken, frog and fish. |
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Crossreactivity: | Does not cross-react with the α1 and β1 subunits in muscle or the neuronal nAChRs. |
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Applications: | IHC (FS), IP, WB
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Recommended Dilutions/Conditions: | Immunohistochemistry (frozen sections, 1:1,000) Western Blot (1:5,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects bands of ~60kDa (γ subunit ) and ~65kDa (δ subunit) by Western blot. Under non-reducing conditions detects a band of ~130kDa (dimer of δ subunit). |
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Formulation: | Liquid. Ascites diluted in PBS containing 0.05% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Structure of nicotinic acetylcholine receptors: A. Karlin; Curr. Opinion Neurobiol.
3, 299 (1993),
Abstract;
Monoclonal antibodies to cytoplasmic domains of the acetylcholine receptor: S.C. Froehner, et al.; J. Biol. Chem.
258, 7112 (1983),
Abstract;
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