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MRP2 monoclonal antibody (M2III-5)

ALX-801-037-C125 125 µg 545.00 USD
ALX-801-037-C250 250 µg 909.00 USD
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Product Details

Alternative Name:Multidrug resistance-associated protein 2, cMOAT, ABCC2
Immunogen:Recombinant C-terminal rat MRP2 (multidrug resistance-associated protein 2; cMOAT) (aa 1339-1541).
UniProt ID:Q63120
Species reactivity:Human, Mouse, Rat
Specificity:Recognizes an internal epitope of MRP2.
Crossreactivity:Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein, MRP1, MRP3 or MRP5.
Applications:ICC, IHC (FS), IHC (PS), WB
Recommended Dilutions/Conditions:Immunocytochemistry: Use 1:20-1:50 dilution on acetone fixed cytospin preparations.
Immunohistochemistry (frozen sections, paraffin sections): Use 1:20 dilution on acetone fixed frozen sections followed by incubation with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex or on paraffin sections after pretreatment with 0.01 M citric acid, pH 6.0 in distilled water at 100°C for 5 min 3 times. After washing, slides can be incubated with biotinylated rabbit anti-mouse IgG and streptavidin conjugated to horseradish peroxidase.
Western Blot: Use 1:20-1:50 dilution and anti-mouse-HRP.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Application Notes:Immunocytochemistry: cytospin preparations
Immunohistochemistry: Does not recognize mouse MRP2 in IHC.
Formulation:Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only

Product Literature References

Boldine enhances bile production in rats via osmotic and Farnesoid X receptor dependent mechanisms: J. Cermanova, et al.; Toxicol. Appl. Pharmacol. 285, 12 (2015), Application(s): Western Blotting, Immunohistochemistry, Abstract;
Maternal bile acid transporter deficiency promotes neonatal demise: Y. Zhang, et al.; Nat. Commun. 6, 8186 (2015), Application(s): Immunoblot analysis of tissue homogenates, Abstract;
Analysis of the in vivo functions of Mrp3: M.G. Belinsky, et al.; Mol. Pharmacol. 68, 160 (2005), Abstract;
Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies: G.L. Scheffer, et al.; Cancer Res. 60, 5269 (2000), Abstract; Full Text
Congenital jaundice in rats with a mutation in a multidrug resistance-associated protein gene: C.C. Paulusma, et al.; Science 271, 1126 (1996), Abstract;

General Literature References

Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines: M. Kool, et al.; Cancer Res. 57, 3537 (1997), Abstract;

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