Product Details
Alternative Name: | Multidrug resistance-associated protein 5, ABCC5 |
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Clone: | M5II-54 |
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Host: | Rat |
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Isotype: | IgG2a |
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Immunogen: | Recombinant human MRP5 (multidrug resistance-associated protein 5) (aa 722-911). |
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UniProt ID: | O15440 |
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Species reactivity: | Human
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Specificity: | Recognizes an internal epitope of human MRP5. |
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Crossreactivity: | Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein, MRP1, MRP2 (cMOAT) or MRP3. |
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Applications: | ICC, IHC (FS), WB
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Recommended Dilutions/Conditions: | Immunocytochemistry: Use 1:20-50 dilution on acetone fixed cytospin preparations. Immunohistochemistry: Use 1:20 dilution on acetone fixed frozen sections followed by incubation with rabbit anti-rat IgG and a monoclonal rat APAAP complex. Alternatively, after incubation with primary antibody (use 1:20) and washing, slides can be incubated with biotinylated rabbit anti-rat IgG and streptavidin conjugated to horseradish peroxidase. Western Blot: Use 1:20-50 dilution and anti-rat-HRP. Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Immunocytochemistry: cytospin preparations |
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Formulation: | Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Evaluation of transport of common antiepileptic drugs by human multidrug resistance-associated proteins (MRP1, 2 and 5) that are overexpressed in pharmacoresistant epilepsy: C. Luna-Tortos, et al.; Neuropharmacology
58, 1019 (2010),
Abstract;
Multidrug resistance-associated proteins 3, 4, and 5: P. Borst, et al.; Pflugers Arch.
453, 661 (2007),
Abstract;
Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies: G.L. Scheffer, et al.; Cancer Res.
60, 5269 (2000),
Abstract;
Full Text
General Literature References
Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines: M. Kool, et al.; Cancer Res.
57, 3537 (1997),
Abstract;