Product Details
Alternative Name: | Multidrug resistance-associated protein 5, ABCC5 |
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Clone: | M5I-1 |
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Host: | Rat |
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Isotype: | IgG2a |
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Immunogen: | Recombinant human MRP5 (multidrug resistance-associated protein 5) (aa 82-168). |
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UniProt ID: | O15440 |
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Species reactivity: | Human
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Specificity: | Recognizes an internal epitope of human MRP5. |
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Crossreactivity: | Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein, MRP1, MRP2 (cMOAT) or MRP3. |
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Applications: | Flow Cytometry, ICC, IHC (FS), WB
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Recommended Dilutions/Conditions: | Immunocytochemistry: Use 1:20-1:50 dilution on acetone fixed cytospin preparations. Immunohistochemistry (frozen sections, weak): Use 1:20 dilution on acetone fixed frozen sections followed by incubation with rabbit anti-rat Ig and a monoclonal rat APAAP complex. Alternatively, after incubation with the primary antibody and washing, slides can be incubated with biotinylated rabbit anti-rat Ig and streptavidin conjugated to horseradish peroxidase. Western Blot: Use 1:20-1:50 dilution and anti-rat-HRP. Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Flow Cytometry: weak, cell permeabilization required Immunocytochemistry: cytospin preparations |
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Formulation: | Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide. |
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Shipping: | Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies: G.L. Scheffer, et al.; Cancer Res.
60, 5269 (2000),
Abstract;
Full Text
General Literature References
Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines: M. Kool, et al.; Cancer Res.
57, 3537 (1997),
Abstract;