Product Details
Alternative Name: | Multidrug resistance-associated protein 3, ABCC3 |
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Clone: | M3II-9 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Recombinant human MRP3 (multidrug resistance-associated protein 3) (aa 830-949). |
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UniProt ID: | O15438 |
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Species reactivity: | Human
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Specificity: | Recognizes an internal epitope of human MRP3. |
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Crossreactivity: | Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein, MRP1, MRP2 (cMOAT) or MRP5. Does not cross-react with mouse, rat or guinea pig MRP2. |
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Applications: | Flow Cytometry, ICC, IHC (FS), WB
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Recommended Dilutions/Conditions: | Immunocytochemistry: Use 1:20-1:50 dilution on acetone or formaldehyde fixed cytospin preparations. Immunohistochemistry (frozen sections): Use 1:20 dilution on acetone fixed frozen sections followed by incubation with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex. Alternatively, after incubation with the primary antibody (1:20) and washing, slides can be incubated with biotinylated rabbit anti-mouse IgG and streptavidin conjugated to horseradish peroxidase. Western Blot: Use 1:20-1:50 dilution and anti-mouse HRP. Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Flow Cytometry: weak, cell permeabilization required Immunocytochemistry: cytospin preparations |
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Formulation: | Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide. |
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Shipping: | Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Tissue distribution and induction of human multidrug resistant protein 3: G.L. Scheffer, et al.; Lab. Invest.
82, 193 (2002),
Abstract;
Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies: G.L. Scheffer, et al.; Cancer Res.
60, 5269 (2000),
Abstract;
Full Text
MRP3, an organic anion transporter able to transport anti-cancer drugs: M. Kool, et al.; PNAS
96, 6914 (1999),
Abstract;
Full Text
General Literature References
Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines : M. Kool, et al.; Cancer Res.
57, 3537 (1997),
Abstract;