Product Details
| Alternative Name: | Multidrug resistance-associated protein 1, ABCC1 |
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| Clone: | MRPm6 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant human MRP1 (multidrug resistance-associated protein 1) (aa 1294-1531). |
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| UniProt ID: | P33527 |
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| Species reactivity: | Human
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| Specificity: | Recognizes the internal epitope (P1511SDLLQQRGL1520) of human MRP1. |
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| Crossreactivity: | Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein or human MRP2 (cMOAT) to MRP5. |
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| Applications: | Flow Cytometry, ICC, IHC (FS), WB
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| Recommended Dilutions/Conditions: | Suggested starting dilution is 1:20
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Flow Cytometry: cell permeabilization required
Immunocytochemistry: cytospin preparations
Immunohistochemistry: Suitable for frozen sections, weak in paraffin sections. |
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| Formulation: | Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Epitope Mapping of MAbs MRPr1, QCRL-1 & MRPm6. Topological model of MRPdepicting the approx. locations of the MRPr1, QCRL-1 and MRPm6 epitopes. The model shown is only one of several possible configurations. MSD=membrane spanning domain; NBD=nucleotide binding domain. From R. Hipfner, et al.; Br. J. Cancer 78,1134 (1998).
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Product Literature References
Flavonoid dimers are highly potent killers of multidrug resistant cancer cells overexpressing MRP1: L. Dury, et al.; Biochem. Pharmacol.
124, 10 (2017),
Application(s): Western blot, BHK-21 cells,
Abstract;
Localization of MRP-1 to the outer mitochondrial membrane by the chaperone protein HSP90β: E. Roundhill, et al.; FASEB J.
30, 1712 (2016),
Abstract;
Dimethyl sulfoxide induces chemotherapeutic resistance in the treatment of testicular embryonal carcinomas: H. Kita, et al.; Oncol. Lett.
10, 661 (2015),
Application(s): Western blot,
Abstract;
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Flow cytometric assessment of P-glycoprotein and multidrug resistance-associated protein activity and expression in canine lymphoma: B. Pozniak, et al.; In Vivo
29, 149 (2015),
Abstract;
Evaluation of transport of common antiepileptic drugs by human multidrug resistance-associated proteins (MRP1, 2 and 5) that are overexpressed in pharmacoresistant epilepsy: C. Luna-Tortos, et al.; Neuropharmacology
58, 1019 (2010),
Abstract;
(R)- and (S)-Verapamil Differentially Modulate the Multidrug-resistant Protein MRP1: T. Perrotton, et al.; J. Biol. Chem.
282, 31542 (2007),
Abstract;
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Cytofluorimetric analysis of a renal tubular cell line and its resistant counterpart: A. Rosati, et al.; Anticancer Res.
20, 3403 (2000),
Abstract;
Expression of the multidrug resistance-associated protein in myelodysplastic syndromes: S. Poulain, et al.; Br. J. Haematol.
110, 591 (2000),
Abstract;
Rhodamine 123 binds to multiple sites in the multidrug resistance protein (MRP1): R. Daoud, et al.; Biochemistry
39, 15344 (2000),
Abstract;
Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies: G.L. Scheffer, et al.; Cancer Res.
60, 5269 (2000),
Abstract;
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The multidrug resistance protein is photoaffinity labeled by a quinoline-based drug at multiple sites: R. Daoud, et al.; Biochemistry
39, 6094 (2000),
Abstract;
Evidence for the involvement of the glutathione pathway in drug resistance in AML: J.M. Sargent, et al.; Adv. Exp. Med. Biol.
457, 205 (1999),
Abstract;
Increased expression of multidrug resistance related proteins Pgp, MRP1, and LRP/MVP occurs early in colorectal carcinogenesis: G.A. Meijer, et al.; J. Clin. Pathol.
52, 450 (1999),
Abstract;
Epitope mapping of monoclonal antibodies specific for the 190-kDa multidrug resistance protein (MRP): D.R. Hipfner, et al.; Br. J. Cancer
78, 1134 (1998),
Abstract;
Overexpression of lung-resistance protein and increased P-glycoprotein function in acute myeloid leukaemia cells predict a poor response to chemotherapy and reduced patient survival: A.G. Borg, et al.; Br. J. Haematol.
103, 1083 (1998),
Abstract;
Optimal immunocytochemical and flow cytometric detection of P-gp, MRP and LRP in childhood acute lymphoblastic leukemia: M.L. Den Boer, et al.; Leukemia
11, 1078 (1997),
Abstract;
Expression of the multidrug resistance-associated protein (MRP) gene in primary non-small-cell lung cancer: K. Nooter, et al.; Ann. Oncol.
7, 75 (1996),
Abstract;
Tissue distribution of the multidrug resistance protein: M.J. Flens, et al.; Am. J. Pathol.
148, 1237 (1996),
Abstract;
Expression of the multidrug resistance-associated protein (MRP) gene in human cancers: K. Nooter, et al.; Clin. Cancer Res.
1, 1301 (1995),
Abstract;
Membrane transport proteins associated with drug resistance expressed in human melanoma: D. Schadendorf, et al.; Am. J. Pathol.
147, 1545 (1995),
Abstract;
Immunochemical detection of the multidrug resistance-associated protein MRP in human multidrug-resistant tumor cells by monoclonal antibodies: M.J. Flens, et al.; Cancer Res.
54, 4557 (1994),
Abstract;
The human multidrug resistance-associated protein MRP is a plasma membrane drug-efflux pump: G.J. Zaman, et al.; PNAS
91, 8822 (1994),
Abstract;
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