Product Details
| Alternative Name: | Multidrug resistance-associated protein 1, ABCC1 |
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| Clone: | MRPm5 |
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| Host: | Mouse |
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| Isotype: | IgG2 |
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| Immunogen: | Recombinant human MRP1 (multidrug resistance-associated protein 1) (aa 986-1204). |
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| UniProt ID: | P33527 |
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| Species reactivity: | Human
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| Specificity: | Recognizes an internal epitope of human MRP1, overexpressed in various human non-P-glycoprotein MDR tumor cell lines. |
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| Crossreactivity: | Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein or human MRP2 (cMOAT) to MRP5. |
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| Applications: | Flow Cytometry, ICC, IHC (FS), WB
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| Recommended Dilutions/Conditions: | Suggested starting dilution is 1:20
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Flow Cytometry: cell permeabilization required
Immunocytochemistry: cytospin preparations
Immunohistochemistry: Suitable for frozen sections, weak in paraffin sections. |
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| Formulation: | Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
2-Cl-IB-MECA regulates the proliferative and drug resistance pathways, and facilitates chemosensitivity in pancreatic and liver cancer cell lines: J. Kotulova, et al.; Int. J. Mol. Med.
49, 31 (2022),
Abstract;
2‑Cl‑IB‑MECA regulates the proliferative and drug resistance pathways, and facilitates chemosensitivity in pancreatic and liver cancer cell lines: J. Kotulova, et al.; Int. J. Mol. Med.
31, 3892 (2022),
Abstract;
Bioluminescent imaging of ABCG2 efflux activity at the blood-placenta barrier: J.S. Kumar, et al.; Sci. Rep.
6, 20418 (2016),
Application(s): Western blot,
Abstract;
Sulindac sulfide selectively increases sensitivity of ABCC1 expressing tumor cells to doxorubicin and glutathione depletion: J.D. Whitt, et al.; J. Biomed. Res.
30, 120 (2016),
Application(s): Immunoblotting assays,
Abstract;
Full Text
Identification and characterization of functionally important elements in the multidrug resistance protein 1 COOH-terminal region: C.J. Westlake, et al.; J. Biol. Chem.
279, 53571 (2004),
Abstract;
Full Text
Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies: G.L. Scheffer, et al.; Cancer Res.
60, 5269 (2000),
Abstract;
Full Text
Optimal immunocytochemical and flow cytometric detection of P-gp, MRP and LRP in childhood acute lymphoblastic leukemia: M.L. Den Boer, et al.; Leukemia
11, 1078 (1997),
Abstract;
Expression of the multidrug resistance-associated protein (MRP) gene in primary non-small-cell lung cancer: K. Nooter, et al.; Ann. Oncol.
7, 75 (1996),
Abstract;
Tissue distribution of the multidrug resistance protein: M.J. Flens, et al.; Am. J. Pathol.
148, 1237 (1996),
Abstract;
Membrane transport proteins associated with drug resistance expressed in human melanoma: D. Schadendorf, et al.; Am. J. Pathol.
147, 1545 (1995),
Abstract;
Immunochemical detection of the multidrug resistance-associated protein MRP in human multidrug-resistant tumor cells by monoclonal antibodies: M.J. Flens, et al.; Cancer Res.
54, 4557 (1994),
Abstract;
The human multidrug resistance-associated protein MRP is a plasma membrane drug-efflux pump: G.J.R. Zaman, et al.; PNAS
91, 8822 (1994),
Abstract;
Full Text
Overexpression of a transporter gene in a multidrug-resistant human lung cancer cell line: S.P. Cole, et al.; Science
258, 1650 (1992),
Abstract;
