For a smaller pack size, please see Prod. No.
ALX-746-005.
CpG oligodeoxynucleotide (Type A) with mixed phosphodiester/phosphorothioate backbone. Specific ligand for human TLR9 (Toll-like receptor 9).
Product Details
Sequence: | 5’-ggGGGACGATCGTCgggggg-3’(lower case letters indicate phosphorothioate linkage). |
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MW: | 6644 (ammonium salt) |
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Source: | Synthetic. |
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Quantity: | 151.1nmol |
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Formulation: | Lyophilized. Sterile. |
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Purity Detail: | Activity and endotoxin tested - TLRGRADE®. |
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Endotoxin Content: | <0.002 EU/µg (LAL test; BioWhittaker) |
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Reconstitution: | For a 200µM stock solution, dissolve the total vial content in 756µl endotoxin-free ddWater (Prod. No. ALX-505-008) or endotoxin-free PBS (Prod. No. ALX-505-007). To obtain optimal dissolving we recommend the following procedure:
- Add 50% of the solvent and let dissolve for 10 min.
- Add remaining 50% of the solvent and mix thoroughly.
- Moderate warming may aid dissolving. |
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Shipping: | Ambient |
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Long Term Storage: | +4°C |
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Use/Stability: | Aqueous stock solution is stable for 1 day when stored at +4°C. |
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Handling: | Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
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Technical Info/Product Notes: | Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008). For the inactive control compound, please see Prod. No. ALX-746-205. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Human blood myeloid and plasmacytoid dendritic cells cross activate each other and synergize in inducing NK cell cytotoxicity: J.J.P. van Beek, et al.; Oncoimmunology
5, e1227902 (2016),
Application(s): Human Dendritic Cells stimulation,
Abstract;
Full Text
CpG-A and CpG-B oligonucleotides differentially enhance human peptide-specific primary and memory CD8+ T-cell responses in vitro: S. Rothenfusser, et al.; Blood
103, 2162 (2004),
Abstract;
Full Text
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